著者
原田 久 ハラダ ヒサシ Hisashi Harada
雑誌
立教法学
巻号頁・発行日
vol.103, pp.73-105, 2020-10-31
著者
原田 久 ハラダ ヒサシ Hisashi Harada
雑誌
立教法学
巻号頁・発行日
vol.107, pp.182-199, 2022-10-31
著者
Hisashi HARADA Kazuhisa KITAZAKI Takeshi TSUJINO Yasuhiro WATARI Sachiyo IWATA Hidemi NONAKA Takeshi HAYASHI Tatsuya TAKESHITA Kanehisa MORIMOTO Mitsuhiro YOKOYAMA
出版者
The Japanese Society of Hypertension
雑誌
Hypertension Research (ISSN:09169636)
巻号頁・発行日
vol.23, no.3, pp.277-284, 2000 (Released:2006-08-10)
参考文献数
34
被引用文献数
20 26

Taurine is known to lower blood pressure in essential hypertension and some experimental hypertensive models. Taurine has also been reported to activate aldehyde dehydrogenase and to inhibit the elevation of plasma acetaldehyde concentration after ethanol intake. Because acetaldehyde, the first metabolite of ethanol, is suspected to be responsible for many adverse effects of alcohol consumption, we examined the effect of taurine supplementation on ethanol-induced hypertension and abnormalities in the intracellular cation metabolism in Witar-Kyoto rats. In Study 1, systolic blood pressure and intraplatelet free calcium were significantly higher in rats who received 15% ethanol in drinking water than in control rats. Oral taurine supplementation (1% taurine and 15% ethanol in drinking water) completely prevented the development of ethanol-induced hypertension. Intraerythrocyte sodium and intraplatelet free calcium were significantly decreased in taurine-supplemented rats as compared with rats who received 15% ethanol only. In Study 2, hemoglobin-associated acetaldehyde (HbAA) was measured as a marker of protein-bound acetaldehyde. HbAA was significantly elevated in rats who received 5% ethanol in drinking water as compared with control rats. Taurine supplementation (1% taurine and 5% ethanol in drinking water) significantly decreased HbAA. Our findings suggest that the oral supplementation of taurine prevents ethanol-induced hypertension by decreasing protein bound acetaldehyde and altering the cation handling by the membrane. (Hypertens Res 2000; 23: 277-284)
著者
Motoyasu Otani Kosuke Kitayama Hiroki Ishikuro Jun-ichiro Hattan Takashi Maoka Hisashi Harada Yuko Shiotani Akane Eguchi Eiji Nitasaka Norihiko Misawa
出版者
Japanese Society for Plant Biotechnology
雑誌
Plant Biotechnology (ISSN:13424580)
巻号頁・発行日
vol.38, no.2, pp.219-226, 2021-06-25 (Released:2021-06-25)
参考文献数
42
被引用文献数
2

Ipomoea obscura, small white morning glory, is an ornamental plant belonging to the family Convolvulaceae, and cultivated worldwide. I. obscura generates white petals including a pale-yellow colored star-shaped center (flower vein). Its fully opened flowers were known to accumulate trace amounts of carotenoids such as β-carotene. In the present study, the embryogenic calli of I. obscura, were successfully produced through its immature embryo culture, and co-cultured with Agrobacterium tumefaciens carrying the β-carotene 4,4′-ketolase (crtW) and β-carotene 3,3′-hydroxylase (crtZ) genes for astaxanthin biosynthesis in addition to the isopentenyl diphosphate isomerase (idi) and hygromycin resistance genes. Transgenic plants, in which these four genes were introduced, were regenerated from the infected calli. They generated bronze (reddish green) leaves and novel petals that exhibited a color change from pale-yellow to pale-orange in the star-shaped center part. Especially, the color of their withered leaves changed drastically. HPLC-PDA-MS analysis showed that the expanded leaves of a transgenic line (T0) produced astaxanthin (5.2% of total carotenoids), adonirubin (3.9%), canthaxanthin (3.8%), and 3-hydroxyechinenone (3.6%), which indicated that these ketocarotenoids corresponded to 16.5% of the total carotenoids produced there (530 µg g−1 fresh weight). Furthermore, the altered traits of the transgenic plants were found to be inherited to their progenies by self-crossing.