- 著者
- Kazuhiro Fujita Megumi Suzuki Kazushi Mizukoshi Yushi Takahashi Toshiaki Yokozeki Izumi Yoshida Mari Maeda-Yamamoto
- 出版者
- Japanese Society for Food Science and Technology
- 雑誌
- Food Science and Technology Research (ISSN:13446606)
- 巻号頁・発行日
- pp.FSTR-D-23-00135, (Released:2023-12-20)
β-Conglycinin (β-CG), a major soy protein, has been associated with the reduction of body fat and triglycerides in the blood. Quantitation of the α, α¢, and β subunits in β-CG is desirable to establish the functional properties of β-CG in soybean. Therefore, in this study, we used the Jess System, a capillary electrophoresis-based immunoassay system, for quantifying β-CG in soybean foods. The validity of this method was confirmed using soybeans and processed soy milk. The within-laboratory reproducibility of the total β-CG amount was < 15 %, and its trueness was > 80 %. The β-CG content in the commercially available soybeans, Kori-tofu, soy milk, soy yogurt, Okara powder, and soy meat was determined to be 40.3–148.5, 65.2–97.2, 6.1–7.8, 3.5, 18.0, and 57.5 mg/g, respectively. For the first time in the literature, this method enables the quantitation of individual subunits of β-CG, despite the large variation in the amount of each subunit.
- 著者
- Honami MORI Yutaka KURODA Shigeki IMAMURA Akira TOYODA Izumi YOSHIDA Masanobu KAWAKAMI Kaoru TABEI
- 出版者
- The Japanese Society of Internal Medicine
- 雑誌
- Internal Medicine (ISSN:09182918)
- 巻号頁・発行日
- vol.42, no.8, pp.665-669, 2003 (Released:2006-03-27)
- 参考文献数
- 20
- 被引用文献数
- 37 56
Objective High-dose trimethoprim-sulfamethoxazole (TMP-SMX) is known to cause hyperkalemia by blocking amiloride-sensitive sodium (Na) channels in distal nephrons. The purpose of this study was to establish whether the standard dose of TMP-SMX could cause electrolyte disorders. Methods and Patients Serum Na, potassium (K) and creatinine (Cr) levels were examined retrospectively in 53 of 77 patients prescribed TMP-SMX, before and after taking the antibiotic combination. Results Electrolyte disorders (Na <135 mEq/l and/or K>5.0 mEq/l) were found in 14 of the 53 patients (26.4%) during TMP-SMX treatment. The average dose was 145.7±24.9 mg/day. The dose of TMP was significantly larger in patients with electrolyte disorders (267.7±84.2 mg vs. 101.9±9.38 mg, p=0.0024). Electrolyte disorders were also seen in 9.1% and 22.2% of patients given the low dose (TMP <80 mg) or standard dose (TMP 80-120 mg) of TMP-SMX, respectively. Electrolyte disorders were seen in 85.7% of patients with renal dysfunction (Cr >1.2 mg/dl), compared with 17.5% of patients with normal renal function (p=0.0008). Logistic regression analysis showed that the dose of TMP and the presence of renal dysfunction increased the incidence of electrolyte disorders with an odds ratio of 2.35 and 80.29, respectively. Conclusion Electrolyte disorders, particularly hyperkalemia and hyponatremia can be detected in patients given TMP-SMX. These disorders are more frequent in patients given high doses, but can also be detected after low-dose administration. Renal dysfunction accelerates the incidence of electrolyte disorders induced by TMP-SMX.(Internal Medicine 42: 665-669, 2003)