- 著者
- Yan YANG LiBin YE JingSong ZHANG YanFang LIU QingJiu TANG
- 出版者
- Japan Society for Bioscience, Biotechnology, and Agrochemistry
- 雑誌
- Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)
- 巻号頁・発行日
- vol.73, no.1, pp.134-139, 2009-01-23 (Released:2009-01-23)
- 参考文献数
- 25
- 被引用文献数
- 34
PISP1, a heteropolysaccharide isolated from fruiting bodies of Phellinus igniarius by hot aqueous extraction and purified by DEAE-Sepharose anion-exchange and gel filtration chromatography, is composed of fucose, galactose, mannose, and 3-O-Me-galactose in a ratio of 1:2:1:2. Methylation, monosaccharide analysis, and NMR studies (1H NMR, 13C NMR, COSY-45°, TOCSY, ROESY, HSQC, and HMBC) revealed that PISP1 had a backbone consisting of 1,6-disubstituted-3-O-Me-α-D-galactopyranosyl residue, 1,3,6-trisubstituted-α-D-manopyranosyl residue, 1,4-disubstituted-α-D-galactopyranosyl residue, and 1,2-disubstituted-α-D-galactopyranosyl residue, and had a 1-substituted-α-L-fucopyranosyl terminal attached to O-3 of a manopyranosyl residue. Preliminary bioactivity tests conducted in vitro revealed that PISP1 stimulated the proliferation of mouse spleen lymphocytes.