Ceek.jp Altmetrics (α ver.)
  1. ホーム
  2. 文献一覧: Jun Kawamoto (著者)
  3. 2件

2 0 0 0 OA Design of the N-Terminus Substituted Curvature-Sensing Peptides That Exhibit Highly Sensitive Detection Ability of Bacterial Extracellular Vesicles

著者
Kenichi Kawano Fumiaki Yokoyama Kouhei Kamasaka Jun Kawamoto Takuya Ogawa Tatsuo Kurihara Shiroh Futaki
出版者
The Pharmaceutical Society of Japan
雑誌
Chemical and Pharmaceutical Bulletin (ISSN:00092363)
巻号頁・発行日
vol.69, no.11, pp.1075-1082, 2021-11-01 (Released:2021-11-01)
参考文献数
38
被引用文献数
4
Extracellular vesicles (EVs) have emerged as important targets in biological and medical studies because they are involved in diverse human diseases and bacterial pathogenesis. Although antibodies targeting the surface biomarkers are widely used to detect EVs, peptide-based curvature sensors are currently attracting an attention as a novel tool for marker-free EV detection techniques. We have previously created a curvature-sensing peptide, FAAV and applied it to develop a simple and rapid method for detection of bacterial EVs in cultured media. The method utilized the fluorescence/Förster resonance energy transfer (FRET) phenomenon to achieve the high sensitivity to changes in the EV amount. In the present study, to develop a practical and easy-to-use approach that can detect bacterial EVs by peptides alone, we designed novel curvature-sensing peptides, N-terminus-substituted FAAV (nFAAV) peptides. The nFAAV peptides exerted higher α-helix-stabilizing effects than FAAV upon binding to vesicles while maintaining a random coil structure in aqueous solution. One of the nFAAV peptides showed a superior binding affinity for bacterial EVs and detected changes in the EV amount with 5-fold higher sensitivity than FAAV even in the presence of the EV-secretory bacterial cells. We named nFAAV5, which exhibited the high ability to detect bacterial EVs, as an EV-sensing peptide. Our finding is that the coil–α-helix structural transition of the nFAAV peptides serve as a key structural factor for highly sensitive detection of bacterial EVs.

1 0 0 0 OA Retraction: Nar is the dominant dissimilatory nitrate reductase under high pressure conditions in the deep-sea denitrifier Pseudomonas sp. MT-1

著者
Yuji Oikawa Yui Sinmura Hikari Ishizaka Ryota Midorikawa Jun Kawamoto Tatsuo Kurihara Chiaki Kato Koki Horikoshi Hideyuki Tamegai
出版者
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
雑誌
The Journal of General and Applied Microbiology (ISSN:00221260)
巻号頁・発行日
vol.61, no.1, pp.10-14, 2015-02-27 (Released:2015-03-31)
参考文献数
24
被引用文献数
3
This article was retracted.