- 著者
-
KANEMITSU HIDEAKI
FUKUDA MASAO
YANO KENJI
- 出版者
- Society of Fermentation Technology, Japan
- 雑誌
- Journal of fermentation technology
- 巻号頁・発行日
- vol.58, no.3, pp.175-181, 1980
The genetic basis of biodegradation of toluene and etylbenzene was studied in a pseudomonad, Pseudomonas species RMT2001,belonging to the fluorescent group. RMT2001 utilized toluenen and ethylbenzene. The degradative ability on toluene exhibited by RMT2001 was lost spontaneously to some extans. Treatment with various curing agents stimulated the rate of eliminaiton. Cells which lost the degradative ability on toluene no longer showed the assimilating ablities on toluene and ethylbenzene originally exhibited by the parent strain. No revertant to a prototype was obtained hitherto. The degradative albility on toluene could be transferred to P. aeruginosa PAO38 by mating at an elevated temperature where the restriction mechanism of PAO38 could be depressed. Transconjugants, however, were extremenly unstable to maintain the phenotype. Transconjugants which were obtained by mating between RMT2001 (donor) and P. putida AC783 (recipient) were stable to maintain the phenotype and showed the degradative abilities on toluene and ethylbenzene exhibited by RMT2001. These facts suggest that degradative abilities on toluene and ethylbenzene in the pseudojmonad were by plasmid DNA.