著者

Aoki Wataru Watanabe Maiko Watanabe Masaki Kobayashi Naoki Terajima Jun Sugita-Konishi Yoshiko Kondo Kazunari Hara-Kudo Yukiko

出版者

日本遺伝学会

雑誌

Genes & Genetic Systems (ISSN:13417568)

巻号頁・発行日

vol.95, no.3, pp.133-139, 2020

被引用文献数

3

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<p><i>Entoloma sarcopum</i> is widely known as an edible mushroom but appears morphologically similar to the poisonous mushrooms <i>E. rhodopolium</i> sensu lato (s. l.) and <i>E. sinuatum</i> s. l. Many cases of food poisoning caused by eating these poisonous mushrooms occur each year in Japan. Therefore, they were recently reclassified based on both morphological and molecular characteristics as sensu stricto species. In this study, we analyzed the nucleotide sequences of the rRNA gene (rDNA) cluster region, mainly including the internal transcribed spacer regions and mitochondrial cytochrome oxidase 1 (CO1) gene, in <i>E. sarcopum</i> and its related species, to evaluate performances of these genes as genetic markers for identification and molecular phylogenetic analysis. We found that the CO1 gene contained lineage-specific insertion/deletion sequences, and our CO1 tree yielded phylogenetic information that was not supported by analysis of the rDNA cluster region sequence. Our results suggested that the CO1 gene is a better genetic marker than the rDNA cluster region, which is the most widely used marker for fungal identification and classification, for discrimination between edible and poisonous mushrooms among Japanese <i>E. sarcopum</i> and related species. Our study thus reports a new genetic marker that is useful for detection of Japanese poisonous mushrooms, <i>Entoloma</i>.</p>

著者

Sasaki Hideki Akiyama Hiroshi Yoshida Yoshifumi KONDO Kazunari AMAKURA Yoshiaki KASAHARA Yoshimasa MAITANI Tamio

出版者

公益社団法人日本薬学会

雑誌

Biological & pharmaceutical bulletin (ISSN:09186158)

巻号頁・発行日

vol.29, no.12, pp.2514-2518, 2006-12-01

被引用文献数

4 20

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In autumn 2004, many Japanese patients with renal failure developed cryptogenic encephalopathy by consuming sugihiratake mushroom, a Japanese delicacy. To elucidate the relationship between the cryptogenic cases and this mushroom, we conducted a multivariate analysis of metabolites in 'Probably Toxic' sugihiratake collected from the area of encephalopathy outbreaks, and 'Probably Safe' sugihiratake collected from unaffected areas using UPLC/ToF MS. The results indicate that the presence of milligram quantities of vitamin D-like compounds per 10g of dried sugihiratake from the areas of encephalopathy outbreaks. Two hypotheses to induce the encephalopathy are proposed: the found metabolites are (1) vitamin D agonists, which induce acute and severe hypercalcemia and/or hyperammonemia and/or vitamin D toxicity, or (2) vitamin D antagonists, which induce acute and severe hypocalcemia.