- 著者
- Yoshio KIKU Yuya NAGASAWA Fuyuko TANABE Kazue SUGAWARA Atsushi WATANABE Eiji HATA Tomomi OZAWA Kei-ichi NAKAJIMA Toshiro ARAI Tomohito HAYASHI
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.78, no.9, pp.1505-1510, 2016 (Released:2016-10-01)
- 参考文献数
- 27
- 被引用文献数
- 15 18
Staphylococcus aureus (SA) is a major cause of bovine mastitis, but its pathogenic mechanism remains poorly understood. To evaluate the role of lipoteichoic acid (LTA) in the immune or inflammatory response of SA mastitis, we investigated the gene expression profile in bovine mammary epithelial cells stimulated with LTA alone or with formalin-killed SA (FKSA) using cap analysis of gene expression. Seven common differentially expressed genes related to immune or inflammatory mediators were up-regulated under both LTA and FKSA stimulations. Three of these genes encode chemokines (IL-8, CXCL6 and CCL2) functioning as chemoattractant molecules for neutrophils and macrophages. These results suggest that the initial inflammatory response of SA infection in mammary gland may be related with LTA induced chemokine genes.
1 0 0 0 OA The bacterial load in milk is associated with clinical severity in cases of bovine coliform mastitis
- 著者
- Yuya NAGASAWA Yoshio KIKU Kazue SUGAWARA Takahiro YABUSAKI Kazuyoshi OONO Kento FUJII Takahide SUZUKI Koji MAEHANA Tomohito HAYASHI
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.81, no.1, pp.107-112, 2019 (Released:2019-01-25)
- 参考文献数
- 43
- 被引用文献数
- 13
We evaluated the relationship between the severity of coliform mastitis and bacterial load in 106 quarter milk samples. We found no significant relationship between somatic cell count and coliform bacterial load in milk in bovine clinical coliform mastitis. Results of the Cochran-Armitage test for trend in milk bacterial load proportions indicated a significant decreasing low group (P<0.001), increasing medium group (P<0.002) and increasing high group (P<0.02) with increasing clinical grade. The present study indicates that the coliform bacterial load in milk is significantly associated with clinical severity states in cases of bovine coliform mastitis, and can be a useful indicator for optimal management of this disease.