著者

Hiroaki Tanaka Yu Suzuki Shotaro Yamasaki Koichiro Yoshino Ko Kato Satoshi Nakamura

出版者

Information Processing Society of Japan

雑誌

IPSJ Transactions on Bioinformatics (ISSN:18826679)

巻号頁・発行日

vol.11, pp.14-23, 2018 (Released:2018-07-05)

参考文献数

43

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Protein production in plants is a hot topic because there are many benefits relative to bacteria, yeasts, and animals, but the amount of protein expression in plants is less. It is argued that editing 5'UTRs increases the amount of translated proteins. However, obtaining such 5'UTRs is difficult due to the cost, time and effort required in experiments. To solve this, we predict the amount of translated proteins by machine learning. In this paper, we propose a method, named “R-STEINER, ” that generates 5'UTRs that increase the amount of proteins of a given gene. The proposed process involves building a model for predicting the amount of translated proteins, generating 5'UTRs, selecting them and increasing the proteins according to the model. This method enables us to obtain 5'UTRs that increase the amount of translated proteins without real synthesis experiments, resulting in reduced cost, time and effort. In our study, we built a prediction model for Oryza sativa and synthesized the 5'UTRs generated by R-STEINER. We confirmed that the model can predict the amount of translated proteins with a correlation coefficient of 0.89.

著者

Takeshi Matsui Eiji Takita Seika Oiwa Asuka Yokoyama Ko Kato Kazutoshi Sawada

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

pp.21.0414a, (Released:2021-06-12)

参考文献数

47

被引用文献数

2

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Plant-made oral vaccines can be a cost-effective method to control infectious diseases of humans and farm animals. Pig edema is a bacterial disease caused by enterohemorrhagic Escherichia coli producing the toxin Shiga toxin 2e (Stx2e). In our previous report, we chose the non-toxic B subunit of Stx2e (Stx2eB) as a vaccine antigen, and Stx2eB was expressed in lettuce (Lactuca sativa L., cv. Green wave). We found that a double repeated Stx2eB (2×Stx2eB) accumulates to higher levels than a single Stx2eB. In this study, we analyzed progeny plants introduced with 2×Stx2eB in which the gene was expressed under the control of conventional cauliflower mosaic virus 35S RNA (CaMV 35S) promoter, and found that the lettuce underwent transgene silencing and bore few seeds. We resolved these problems by using a transgene cassette which harbored a transcriptional promoter derived from the lettuce ubiquitin gene and a longer version of HSPT. The lettuce harboring this expression construct will be valuable in establishing the seed lot system on the basis that thousands of seeds can be obtained from one plant body and the resulting progeny plants accumulate 2×Stx2eB at high levels without the transgene silencing.

著者

Takeshi Matsui Eiji Takita Seika Oiwa Asuka Yokoyama Ko Kato Kazutoshi Sawada

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.2, pp.239-246, 2021-06-25 (Released:2021-06-25)

参考文献数

47

被引用文献数

2

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Plant-made oral vaccines can be a cost-effective method to control infectious diseases of humans and farm animals. Pig edema is a bacterial disease caused by enterohemorrhagic Escherichia coli producing the toxin Shiga toxin 2e (Stx2e). In our previous report, we chose the non-toxic B subunit of Stx2e (Stx2eB) as a vaccine antigen, and Stx2eB was expressed in lettuce (Lactuca sativa L., cv. Green wave). We found that a double repeated Stx2eB (2×Stx2eB) accumulates to higher levels than a single Stx2eB. In this study, we analyzed progeny plants introduced with 2×Stx2eB in which the gene was expressed under the control of conventional cauliflower mosaic virus 35S RNA (CaMV 35S) promoter, and found that the lettuce underwent transgene silencing and bore few seeds. We resolved these problems by using a transgene cassette which harbored a transcriptional promoter derived from the lettuce ubiquitin gene and a longer version of HSPT. The lettuce harboring this expression construct will be valuable in establishing the seed lot system on the basis that thousands of seeds can be obtained from one plant body and the resulting progeny plants accumulate 2×Stx2eB at high levels without the transgene silencing.