著者

Hideki INNAN Ryohei TERAUCHI Naohiko T. MIYASHITA Koichiro TSUNEWAKI

出版者

The Genetics Society of Japan

雑誌

遺伝学雑誌 (ISSN:0021504X)

巻号頁・発行日

vol.70, no.2, pp.185-196, 1995 (Released:2004-06-08)

参考文献数

22

被引用文献数

19 35

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In order to investigate the intraspecific variation of Prunus yedoensis (Someiyoshino) and interspecific relationship among P. yedoensis, P. lannesiana (Oshimazakura) and P. pendula (Edohigan), DNA fingerprinting study was conducted by using two different kinds of probes, M13 repeat sequence and (GACA)4 synthetic oligonucleotide. In this study, 68 plants of P. yedoensis grown in 46 prefectures in Japan were investigated. All the P. yedoensis individuals investigated showed the completely same banding pattern, indicating their clonal origin from a single plant. On the other hand, each of P. lannesiana and P. pendula individuals investigated showed a unique banding pattern, suggesting a considerable amount of genetic variation in these two species. About 90% of bands in DNA fingerprints of P. yedoensis were detected in either P. lannesiana or P. pendula. This result supports the hypothesis that P. yedoensis is an interspecific hybrid between P. lannesiana and P. pendula. From those results, it is concluded that P. yedoensis was produced only once through hybridization between P. lannesiana and P. pendula, and that this particular hybrid plant has been spread vegetatively all over Japan.

著者

Koichiro Tsunewaki

出版者

日本育種学会

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

vol.60, no.2, pp.177-178, 2010 (Released:2010-06-09)

被引用文献数

1 2

著者

Hitoshi KIHARA Koichiro TSUNEWAKI

出版者

日本遺伝学会

雑誌

遺伝学雑誌 (ISSN:0021504X)

巻号頁・発行日

vol.37, no.4, pp.310-313, 1962 (Released:2007-05-21)

参考文献数

7

被引用文献数

13 32

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The frequencies of haploids in nucleus-substitution lines of T. vulgare var. erythrospermum and Taylor's Triticale having the cytoplasm of Aegilops caudata, were investigated and compared with those found in the ordinary strains.The haploid frequency of the former was 1.7%, while it amounted to 53% in the latter. No haploids were found in the respective controls. The differences are significant in both cases.This result indicates that an alien cytoplasm will increase the tendency of a species to develop parthenogenetically haploid embryos. The difference between T. vulgare var. erythrospermum and Taylor's Triticale indicates that different genotypes respond differently to the same alien cytoplasm.The use of an alien cytoplasm provides a new means for artificial production of haploids.

著者

Koichiro Tsunewaki Naoki Mori Shigeo Takumi

出版者

日本遺伝学会

雑誌

Genes & Genetic Systems (ISSN:13417568)

巻号頁・発行日

vol.89, no.5, pp.195-202, 2014-10-01 (Released:2015-04-02)

参考文献数

19

被引用文献数

2

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In the course of reconstructing Aegilops caudata from its own genome (CC) and its plasmon, which had passed half a century in common wheat (genome AABBDD), we produced alloplasmic Ae. cylindrica (genome CCDD) with the plasmon of Ae. caudata. This line, designated (caudata)-CCDD, was found to express male sterility in its second substitution backcross generation (SB2) of (caudata)-AABBCCDD pollinated three times with the Ae. cylindrica pollen. We repeatedly backcrossed these SB2 plants with the Ae. cylindrica pollen until the SB5 generation, and SB5F2 progeny were produced by self-pollination of the SB5 plants. Thirteen morphological and physiological characters, including pollen and seed fertilities, of the (caudata)-CCDD SB5F2 were compared with those of the euplasmic Ae. cylindrica. The results indicated that the male sterility expressed by (caudata)-CCDD was due to genetic incompatibility between the Ae. cylindrica genome and Ae. caudata plasmon that did not affect any other characters of Ae. cylindrica. Also, we report that the genome integrity functions in keeping the univalent transmission rate high.