- 著者
- SEIICHI AONUMA FUMIO ARIJI KOTARO OIZUMI KIYOSHI KONNO
- 出版者
- 東北ジャーナル刊行会
- 雑誌
- The Tohoku Journal of Experimental Medicine (ISSN:00408727)
- 巻号頁・発行日
- vol.152, no.2, pp.119-128, 1987 (Released:2006-08-31)
- 参考文献数
- 9
- 被引用文献数
- 2 2
AONUMA, S., ARIJI, F., OIZUMI, K. and KONNO, K. Electron Microscopy of Pseudomonas aeruginosa Treated with Sulbenicillin and Dibekacin. Tohoku J. exp. Med., 1987, 152 (2), 119-128 - A possible mechanism responsible for the combined effects of sulbenicillin and dibekacin on Pseudomonas aeruginosa TAM 1007 was investigated. The bactericidal activity of the above two drugs in combination was very strong. The regrowth of test strains after removal of the drugs was suppressed markedly, even when they were exposed to sulbenicillin plus dibekacin at a subinhibitory concentration of individual drugs. Sulbenicillin caused elongation of the bacterial cells. At the early stage of elongation, no demonstrable changes of ultrastructure of the cell wall were observed. At the late stage, lysis of the peptidoglycan layer occurred and spheroplast was formed. However, most of the outer membrane of the cell wall remained intact. Sulbenicillin acts upon the peptidoglycan layer, but not on the outer membrane. Thus it is difficult for sulbenicillin alone to cause cell lysis. On the other hand, dibekacin caused destruction of ribosomes and lysis of the outer membrane of the cell wall. Both sulbenicillin and dibekacin act on the cell wall, the former on the peptidoglycan layer (the inner membrane) and the latter on the outer membrane. The combined use of sulbenicillin and dibekacin caused elongation of bacilli and severe destruction of the inner and outer membranes of the cell wall. These morphological changes occurred even when the concentration of the individual drug was lower than its minimum inhibitory concentration (MIC). Furthermore, the cells elongated by sulbenicillin were ruptured easily when treated with dibekacin subsequently. The bacilli treated with dibekacin at a concentration lower than MIC and then treated with sulbenicillin at a concentration lower than MIC showed a marked elongation of the cells, which indicated that the effects of sulbenicillin was enhanced by dibekacin. These findings suggested strongly that sulbenicillin and dibekacin act on cell wall constituents and that their effects were complementary and synergistic.
- 著者
- Masahide KAJI Haruko KUNO Toshiomi TURU Yoshihiro SATO Kotaro OIZUMI
- 出版者
- The Japanese Society of Internal Medicine
- 雑誌
- Internal Medicine (ISSN:09182918)
- 巻号頁・発行日
- vol.40, no.7, pp.594-597, 2001 (Released:2006-03-27)
- 参考文献数
- 10
- 被引用文献数
- 20 22
Objective Myocarditis has been described as a complication of influenza. Patients with influenza may have symptoms and abnormal laboratory data (including chest X-ray, electrocardiogram, etc.) suggestive of myocarditis, although few observations have been made regarding the prevalence of asymptomatic myocardial injury. We investigated whether influenza can produce myocardial injury without cardiac symptoms. Methods During the epidemic of influenza A (H3N2) from 1998 to 1999 in Japan, we examined possible cardiac muscle damage associated with influenza in patients without apparent clinical myocardial injury by measuring serum myosin light chain concentrations. Patients Ninety-six influenza-positive patients (46 males and 50 females, average age 43.4 years) without impaired renal function were studied. Results Of these patients, 11 (11.4%) had elevated serum myosin light chain I concentrations. Conclusion Asymptomatic myocardial injury may be present in patients with influenza even when they have no symptoms suggestive of myocardial injury.(Internal Medicine 40: 594-597, 2001)