著者

Ikuo KOBAYASHI Masaru AKITA Masaki TAKASU Teruaki TOZAKI Hironaga KAKOI Kotono NAKAMURA Natsuko SENJU Ryota MATSUYAMA Yoichiro HORII

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.81, no.5, pp.707-711, 2019 (Released:2019-05-11)

参考文献数

23

被引用文献数

11

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The Misaki horse is a Japanese native horse, known as the “feral horse of Cape Toi”. In this study, we acquired the genetic information to establish their studbook, and analyzed their genetic characteristics for conservation. We genotyped 32 microsatellites and a mitochondrial D-loop region in 77 Misaki horses (80.2% of the population). The average number of alleles, observed heterozygosity, and expected heterozygosity were 3.4, 0.509, and 0.497, respectively. A neighbor-joining phylogenetic tree of individuals was constructed. Moreover, the results suggested that Misaki horses experienced a bottleneck, but it was neither severe nor recent. In addition, three mitochondrial haplotypes were confirmed. Consequently, we clarified the genetic background of Misaki horses that have been resident at Cape Toi for a long time.

著者

Shiori GAMO Teruaki TOZAKI Hironaga KAKOI Kei-ichi HIROTA Kotono NAKAMURA Noriko NISHII Julio ALUMUNIA Masaki TAKASU

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.81, no.1, pp.91-94, 2019 (Released:2019-01-08)

参考文献数

15

被引用文献数

4

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A routine parentage test as part of a conservation program for Kiso horses identified a possible sex chromosome anomaly in a 7 months-old filly because of an aberrant result using LEX3, an X-linked marker. We then analyzed X-linked markers (LEX26, TKY38, and TKY270), Y-linked markers (Eca.YH12, Eca.YM2, Eca.YA16, and the sex-determining region Y gene), and an X/Y marker (Amelogenin gene). This analysis demonstrated that the filly had not inherited an X chromosome from her sire. A karyotyping analysis confirmed that the filly was 63,XO. As it was suspected that the horse would be sterile, we avoided using the horse as a broodmare; the information should also serve to prevent unnecessary conflict between owners transferring and receiving the horse.

著者

Mako NAKAMURA Teruaki TOZAKI Hironaga KAKOI Kotono NAKAMURA Reza RAJABI-TOUSTANI Yasunori OHBA Tatsuya MATSUBARA Masaki TAKASU

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0535, (Released:2020-03-09)

被引用文献数

1

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The Kiso horse is native to Japan and is on the verge of extinction. Here, we used microsatellites to characterize changes in their genetic diversity over time. We divided a population of Kiso horses that genotyped during 2007–2017 into three groups based on birth year: Group 1, 1980–1998 (70 horses); Group 2, 1999–2007 (61 horses); and Group 3, 2008–2017 (42 horses). We genotyped 31 microsatellites to calculate average number of alleles, observed heterozygosity, and expected heterozygosity. All indicators decreased across age groups. The results indicate that Kiso horses have been experiencing a drop in genetic diversity, and the population is expected to experience further decline unless appropriate measures are implemented.