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3 0 0 0 OA Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Platform to Study SARS-CoV-2 Related Myocardial Injury

著者
Chun-Ka Wong Hayes Kam-Hei Luk Wing-Hon Lai Yee-Man Lau Ricky Ruiqi Zhang Antonio Cheuk-Pui Wong George Chi-Shing Lo Kwok-Hung Chan Ivan Fan-Ngai Hung Hung-Fat Tse Patrick Chiu-Yat Woo Susanna Kar-Pui Lau Chung-Wah Siu
出版者
The Japanese Circulation Society
雑誌
Circulation Journal (ISSN:13469843)
巻号頁・発行日
pp.CJ-20-0881, (Released:2020-09-26)
参考文献数
10
被引用文献数
33
Background:SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition.Methods and Results:Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-α were upregulated, while ACE2 was downregulated.Conclusions:Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.

1 0 0 0 OA Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Platform to Study SARS-CoV-2 Related Myocardial Injury

著者
Chun-Ka Wong Hayes Kam-Hei Luk Wing-Hon Lai Yee-Man Lau Ricky Ruiqi Zhang Antonio Cheuk-Pui Wong George Chi-Shing Lo Kwok-Hung Chan Ivan Fan-Ngai Hung Hung-Fat Tse Patrick Chiu-Yat Woo Susanna Kar-Pui Lau Chung-Wah Siu
出版者
The Japanese Circulation Society
雑誌
Circulation Journal (ISSN:13469843)
巻号頁・発行日
vol.84, no.11, pp.2027-2031, 2020-10-23 (Released:2020-10-23)
参考文献数
10
被引用文献数
15 33
Background:SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition.Methods and Results:Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-α were upregulated, while ACE2 was downregulated.Conclusions:Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.