著者

Munehiro YOSHIDA Yuka KIMURA Mamiko ABE Tatsuhiko ANDO Hiroshi TACHI Kenji FUKUNAGA

出版者

Center for Academic Publications Japan

雑誌

Journal of Nutritional Science and Vitaminology (ISSN:03014800)

巻号頁・発行日

vol.47, no.3, pp.248-252, 2001 (Released:2009-04-28)

参考文献数

19

被引用文献数

5 7

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For determination of selenium (Se) in biological materials, an improved method based on high performance liquid chromatographic determination of the fluo-rophore formed by reaction of selenite with 2, 3-diaminonapththalene was developed. The concentration detection limits were 0.5 ng/g in dried materials and 0.03 ng/mL in fluid ma-terials. In quadruplicate assays of 11 biological reference materials using the proposed method, measured Se concentrations were not significantly different from their certified val-ues, Thus, the proposed method is reliable and suitable for the determination of trace levels of Se in foods. Using the proposed method, Se concentrations in various kinds of tea were de-termined to assess the contribution of tea to daily Se intake in the Japanese population. Se concentration in the leaves of general black, green and oolong tea obtained in local retail stores was 33±19 ng/g (n=40). The leaves of a particular Chinese green tea sold under the name “high Se tea” were found to contain 455±184 ng/g (n=14) of Se. While the percent-age of Se extractable by infusion was less than 5% for the general teas, that in the high Se tea was more than 20%. These results indicated that intake of tea does not contribute to daily Se intake in the Japanese population. However, since infusions from high Se tea con-tained over 5 ng/mL of Se, consumption of over 1 L/d of tea derived from such high Se teas may increase the daily Se intake by close to 10%.