著者

Marco BOTTINELLI Elisa RAMPACCI Valentina STEFANETTI Maria Luisa MARENZONI Ashley M MALMLOV Mauro COLETTI Fabrizio PASSAMONTI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0543, (Released:2016-01-04)

被引用文献数

7

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Canine herpesvirus-1 (CaHV-1) is a globally distributed pathogen causing reproductive, respiratory, ocular and neurological disorders in adult dogs and neonatal death in puppies. This pathogen is considered poorly immunogenic, and neutralizing antibodies are found for only a short time following exposure. Further, seroprevalence can be affected by several epidemiological factors. A virological survey was conducted in a high-density population breeding kennel in Central Italy. There were several factors predisposing animals to CaHV-1 infection, such as age, number of pregnancies, experience with mating and dog shows, cases of abortion, management, and environmental hygiene. Serum neutralization (SN) and nested PCR assays were used to estimate prevalence of CaHV-1. None of the submitted samples tested positive for nested PCR, and none of the sera tested CaHV-1 positive. No association was observed between antibody titers and risk factors, and no sign of viral reactivation was detected in either males or females. These results suggest that CaHV-1 is not circulating within this kennel and that further studies are needed in order to better understand the distribution of the virus within Italy.

著者

Patrizia Casagrande PROIETTI Valentina STEFANETTI Doreene R. HYATT Maria Luisa MARENZONI Stefano CAPOMACCIO Mauro COLETTI Annalisa BIETTA Maria Pia FRANCIOSINI Fabrizio PASSAMONTI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0043, (Released:2015-04-18)

被引用文献数

22

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Biofilm-forming ability is increasingly being recognized as an important virulence factor in several Staphylococcus species. This study evaluated the biofilm-forming ability of sixty canine derived clinical isolates of S. pseudintermedius, using three phenotypic methods, microtiter plate test (MtP), Congo red agar method (CRA) and tube adherence test, and the presence and impact of biofilm-associated genes (icaA and icaD). The results showed that icaA and icaD genes were detected concomitantly in 55 (91.7%) of 60 isolates. A majority (88.3%) of the strains screened had matching results by the tube adherence test, MtP and PCR analysis. Better agreement (95%) was found between the PCR-based analysis and the CRA. Results of the icaA and icaD gene PCRs showed good agreement with CRA results, with a kappa of 0.7. Comparing the phenotypic methods, the statistical analysis showed that the agreement among the phenotypical tests using categorical data was generally good. Considering two classes (biofilm producer and biofilm non-producer), the percentage of matching results between the CRA method and the tube adherence test and between the CRA method and the MtP was 93.3%. A concordance of 100% was revealed between the MtP and the tube adherence test. The results indicate a high prevalence of the ica genes within S. pseudintermedius isolates, and their presence is associated with in vitro formation of a biofilm. A combination of phenotypic and genotypic tests is recommended for investigating biofilm formation in S. pseudintermedius.