- 著者
- Matsuda Akira Takenuki Kenji Itoh Hiroko Sasaki Takuma Ueda Tohru
- 出版者
- 公益社団法人 日本薬学会
- 雑誌
- CHEMICAL & PHARMACEUTICAL BULLETIN (ISSN:00092363)
- 巻号頁・発行日
- vol.35, no.9, pp.3967-3970, 1987
- 被引用文献数
- 32
We have synthesized 2'-deoxy-2' (S) -methylcytidine (7), a new antileukemic nucleoside. The carbonyl methylation of 2'-ketonucleoside (1) with MeLi, Me<SUB>3</SUB>Al and MeMgX was examined. Only in the reaction with MeMgX, did the more hindered β-attack afford the 2'-methyl-t-alcohol (2b). Compound 2b was converted into the methyl oxalate (4), which was subjected to radical deoxygenation to give the 2'-deoxy-2' (S) -methyl derivative (5). The deprotection of 5 followed by substitution with NH<SUB>3</SUB> furnished 7. The structure-activity relationships of 7 and some other 2'-branched-chain sugar cytidines against L1210 cells are also described.
- 著者
- Kataoka Mayumi Kouda Yasuo Sato Kousuke Minakawa Noriaki Matsuda Akira
- 出版者
- Royal Society of Chemistry
- 雑誌
- Chemical Communications (ISSN:13597345)
- 巻号頁・発行日
- vol.47, no.30, pp.8700-8702, 2011-08-14
- 被引用文献数
- 11
The synthesis of the 3'-deoxyapionucleoside 3"-triphosphates (apioNTPs) having the four natural nucleobases and their enzymatic incorporation into a DNA-DNA primer-template have been tried. Therminator DNA polymerase was shown to incorporate these apioNTPs effectively giving 43mer DNA-apioNA chimera.