- 著者
-
Wu Yuluo
Hinenoya Atsushi
Taguchi Takashi
NAGITA Akira
SHIMA Kensuke
TSUKAMOTO Teizo
SUGIMOTO Norihiko
ASAKURA Masahiro
YAMASAKI Shinji
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.72, no.5, pp.589-597, 2010
- 被引用文献数
-
27
Shiga toxin-producing <i>Escherichia coli</i> (STEC) isolated from Japan were investigated for the distribution of virulence genes. A total of 232 STEC strains including 171 from cattle and 61 from human were examined for the occurrence of genes responsible for bacterial adhesions to intestine, e.g., <i>eae</i> (intimin, <i>E. coli </i>attaching and effacing), <i>saa</i> (STEC autoagglutinating adhesin), <i>iha</i> (<i>irgA</i> homologue adhesin), <i>efa1</i> (<i>E. coli</i> factor for adherence), <i>lpfA</i><i><sub>O113</sub></i> (long polar fimbriae), and <i>ehaA</i> (EHEC autotransporter) by colony hybridization assay. Similarly, the presence of toxigenic <i>cdt</i> (cytolethal distending toxin), and <i>subAB</i> (subtilase cytotoxin) genes were also checked. Among cattle isolates, 170, 163, 161, 155, 112 and 84 were positive for <i>lpfA</i><i><sub>O113</sub></i> (99%), <i>ehaA</i> (95%), <i>iha</i> (94%), <i>saa</i> (91%), <i>subAB</i> (65%), and <i>cdt-V</i> (49%), respectively, while 2 were positive for <i>eae</i> (1.2%) and <i>efa1</i> (1.2%) each. In case of human isolates, 60, 59, 58 and 58 were positive for <i>ehaA</i> (98%), <i>iha</i> (97%), <i>efa1</i> (95%), and <i>eae</i> (95%), respectively, while 11, 2, 2, and 1 were positive for <i>lpfA</i><i><sub>O113</sub></i> (18%), <i>saa</i> (3.3%), <i>cdt-V</i> (3.3%), and <i>subAB</i> (1.6%), respectively. Therefore, in human STEC isolates <i>efa1</i> and <i>eae</i> whereas in cattle isolates <i>saa</i>, <i>lpfA</i><i><sub>O113</sub></i>, <i>cdt-V</i> and <i>subAB</i> were prevalent. These data indicate differential occurrence of some pathogenic genes in human and cattle originated STEC strains in Japan.<br>