- 著者
- Yamaguchi Ryo-taro Hirano-Iwata Ayumi Kimura Yasuo Niwano Michio Miyamoto Ko-ichiro Isoda Hiroko Miyazaki Hitoshi
- 出版者
- American Institute of Physics
- 雑誌
- Applied physics letters (ISSN:00036951)
- 巻号頁・発行日
- vol.91, no.20, pp.203902, 2007-11
- 被引用文献数
- 13
We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% CO2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay.