著者

Othman Mahmoud Ben Han Junkyu Omri Abdelfatteh El Ksouri Riadh Neffati Mohamed Isoda Hiroko

出版者

Hindawi Publishing Corporation

雑誌

Evidence-based complementary and alternative medicine (ISSN:1741427X)

巻号頁・発行日

vol.2013, pp.737401, 2013

被引用文献数

10

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This study aimed to investigate the antistress properties of the ethanol extract of Cymbopogon schoenanthus (CSEE), growing wild in the southern part of Tunisia. The effect of extracts on H2O2-induced cytotoxicity and stress in human neuroblastoma SH-SY5Y cells. Its effect on stress-induced in ICR mice was exposed to force swim and tail suspension, in concordance with heat shock protein expression (HSP27 and HSP90), corticosterone, and catecholamine neurotransmitters level. Our results demonstrated that pretreatment of SH-SY5Y cells with CSEE at 1/2000, 1/1000, and 1/500 v/v dilutions significantly inversed H2O2-induced neurotoxicity. Moreover, CSEE treatments significantly reversed heat shock protein expression in heat-stressed HSP47-transformed cells (42°C, for 90 min) and mRNA expression of HSP27 and HSP90 in H2O2-treated SH-SY5Y. Daily oral administration of 100 mg/kg and 200 mg/kg CSEE was conducted to ICR mice for 2 weeks. It was resulted in a significant decrease of immobility time in forced swimming and tail suspension tests. The effect of CSEE on animal behavior was concordant with a significant regulation of blood serum corticosterone and cerebral cortex levels of catecholamine (dopamine, adrenaline, and noradrenaline). Therefore, this study was attempted to demonstrate the preventive potential of CSEE against stress disorders at in vitro and in vivo levels.

著者

Shimoda Yoichi Han Junkyu Kawada Kiyokazu Smaoui Abderrazak Isoda Hiroko

出版者

Hindawi

雑誌

Journal of biomedicine and biotechnology (ISSN:11107243)

巻号頁・発行日

vol.2012, pp.428514, 2012

被引用文献数

6

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Energy metabolism is a very important process to improve and maintain health from the point of view of physiology. It is well known that the intracellular ATP production is contributed to energy metabolism in cells. Cistus monspeliensis is widely used as tea, spices, and medical herb; however, it has not been focusing on the activation of energy metabolism. In this study, C. monspeliensis was investigated as the food resources by activation of energy metabolism in human intestinal epithelial cells. C. monspeliensis extract showed high antioxidant ability. In addition, the promotion of metabolites of glycolysis and TCA cycle was induced by C. monspeliensis treatment. These results suggest that C. monspeliensis extract has an ability to enhance the energy metabolism in human intestinal cells.

著者

Yamaguchi Ryo-taro Hirano-Iwata Ayumi Kimura Yasuo Niwano Michio Miyamoto Ko-ichiro Isoda Hiroko Miyazaki Hitoshi

出版者

American Institute of Physics

雑誌

Applied physics letters (ISSN:00036951)

巻号頁・発行日

vol.91, no.20, pp.203902, 2007-11

被引用文献数

13

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We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% CO2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay.

著者

Motojima Hideko Yamada Parida Irie Mitsuteru Ozaki Masuo Shigemori Hideyuki Isoda Hiroko

出版者

Springer Japan

雑誌

Journal of material cycles and waste management (ISSN:14384957)

巻号頁・発行日

vol.14, no.3, pp.169-180, 2012-09

被引用文献数

14

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In this study, the main characteristics and soil amendment effects on the saline–alkali soil of humic acid extracted from solubilized excess sludge (SS-HA) were investigated. The excess sludge was solubilized prior to extraction to improve the humic acid recovery rate. The structural features of SS-HA were characterized by an elemental analysis, Fourier transform infrared spectroscopy, and 1H-nuclear magnetic resonance spectroscopy, and compared with those of HA extracted from non-solubilized excess sludge (ES-HA). The results showed that extraction efficiency of humic acid was enhanced by using solubilization, although structural properties of humic acid extracted from solubilized excess sludge were almost the same as those of ES-HA. To study a utilization method of SS-HA, the soil amendment effects on saline–alkali soil by mixture of SS-HA were investigated with a model soil-column experiment. SS-HA reduced the pH of the saline–alkali soil, and the effect was immediately observed or faster than the case in which only peat is added. Moreover, the cation exchangeable capacity of the saline–alkali soil was enhanced by addition of SS-HA.

著者

Yamada Parida Ono Takako Shigemori Hideyuki Han Junkyu Isoda Hiroko

出版者

Springer Science+Business Media B.V.

雑誌

Cytotechnology (ISSN:09209069)

巻号頁・発行日

vol.64, no.3, pp.349-356, 2012-05

被引用文献数

10

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In this study, the anti-allergy potency of thirteen tannins isolated from the galls on buds of Carpinus tschonoskii (including two tannin derivatives) was investigated. RBL-2H3 (rat basophilic leukemia) cells were incubated with these compounds, and the release of β-hexosaminidase and cytotoxicity were measured. Of the thirteen tannins, tetragalloylglucose (2), pentagalloylglucose (3), casuarictin (4), and casuarinin (9) were the most potent inhibitors, and all the tannins showed no cytotoxic effect after 24 h of incubation. The results obtained suggest that tannins from C. tschonoskii are capable of inhibiting allergic reactions and may be useful for the treatment or prevention of type I allergic diseases.

著者

Han Jun Kyu Akutsu Mitsuaki Talorete Terence P. N. Maekawa Takaaki Tanaka Toshiyuki Isoda Hiroko

出版者

Springer

雑誌

Cytotechnology (ISSN:09209069)

巻号頁・発行日

vol.47, no.1-3, pp.89-96, 2005-01

被引用文献数

11 10

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On the basis of transepithelial electrical resistance (TER) measurements, we found that capsaicin (100 μM)-treated human intestinal Caco-2 cells show a momentary increase in tight-junction (TJ) permeability (decrease in TER) followed by a complete recovery. We used proteome analysis to search for proteins that are associated with the recovery of TJ permeability in capsaicin-treated Caco-2 cells. A protein with a relative molecular mass of 14 kDa was found to be expressed more highly in capsaicin-treated cells than in nontreated cells. Mass spectrometry and sequence analyses revealed that the protein that is expressed significantly upon capsaicin treatment is the ribosomal protein P2; its cDNA sequence was identical to that found in the human genome database. An increase in the amount of cellular filamentous actin (F-actin) was shown after 8 h of incubation with capsaicin. It has been reported that P2 activates elongation factor 2, which stabilizes F-actin filaments, and that the depolymerization of F-actin is associated with the increase in TJ permeability (decrease in TER). Consequently, these results suggest that P2 plays an important role in the recovery of the TJ permeability in capsaicin-treated human intestinal cells.