- 著者
- Nobuhito HAYASHIMOTO Hideaki YOSHIDA Kazuo GOTO Akira TAKAKURA
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.70, no.5, pp.493-495, 2008 (Released:2008-06-04)
- 参考文献数
- 12
- 被引用文献数
- 5 10 1
We isolated Streptobacillus moniliformis, the causative agent of rat-bite fever in humans, from the salivary gland of a pet rat postmortem. The isolate was a Gram-negative pleomorphic coccobacillus, which produced acid from glucose and showed enzymatic activities for eight items in the API ZYM system. The results were consistent with those of the reference strain, ATCC 14647T, except for acid production from dextrin. Partial sequencing of 16S rRNA (1,440 bp) and gyrB genes (514 bp) of the isolate revealed similarities of 100% and 99.8%, respectively, to those of S. moniliformis in GenBank. Therefore, the isolate was identified as S. moniliformis. These results suggested the potential risk of rat-bite fever arising from pet rats in Japan.
- 著者
- Kazuo GOTO Mikachi YAMAMOTO Miwa ASAHARA Takashi TAMURA Mitsuru MATSUMURA Nobuhito HAYASHIMOTO Koichi MAKIMURA
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.1204080808, (Released:2012-04-13)
- 被引用文献数
- 3 12
Mycoplasma species identification is based on biochemical, immunological, and molecular methods that require several days for accurate identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a novel method for identification of bacteria and has recently been introduced into the clinical microbiology laboratory as a rapid and accurate technique. This method allows a characteristic mass spectral fingerprint to be obtained from whole inactivated mycoplasmal cells. In this study, we evaluated the performance of the MALDI-TOF MS for the identification of Mycoplasma by comparison with standard sequence analysis of 16S rRNA. We developed the first database of MALDI-TOF MS profiles of Mycoplasma species, containing Mycoplasma pulmonis, M. arthritidis, and M. neurolyticum, which are the most common pathogens in mice and/or rats, and species-specific spectra were recorded. Using the database, 6 clinical isolates were identified. Six tracheal swabs from 4 mice and 2 rats were cultured on PPLO agar for 4 to 7 days, and the colonies were directly applied to analyze the protein profiles. Five strains were identified as M. pulmonis, and 1 strain from a mouse was identified as M. neurolyticum (spectral scores were >2.00); the results were consistent with the results of the 16S rRNA gene sequence analysis (homologies>97.0%). These data indicate that MALDI-TOF MS can be used as a clearly rapid, accurate, and cost-effective method for the identification of M. pulmonis isolates, and this system may represent a serious alternative for clinical laboratories to identify Mycoplasma species.