- 著者
- Alexander I. KUZMENKO Etsuo NIKI Noriko NOGUCHI
- 出版者
- Japan Oil Chemists' Society
- 雑誌
- Journal of Oleo Science (ISSN:13458957)
- 巻号頁・発行日
- vol.50, no.6, pp.497-506, 2001 (Released:2009-07-28)
- 参考文献数
- 21
- 被引用文献数
- 7 13
The antioxidizig action of 20-hydroxyecdysone was investigated in several systems in vitro. Changes in the parameters of chemiluminescence in the presence of 20-hydroxyecdysone were found in rat liver mitochondrial fractions. The effect of 20-hydroxyecdysone on methyl linoleate micelles free radical oxidation was studied. The rate of oxygen uptake in methyl linoleate micelles was found to be smaller when 20-hydroxyecdysone was present than at its absence. The action of 20-hydroxyecdysone in the liposomal membranes oxidation was investigated be means of α-tocopherol consumption. A lower rate of α-tocopherol consumption in the oxidation of liposomal membranes was found when 20-hydroxyecdysone was present. The effect of cholesterol on free radical formation in liposomal membranes was investigated. The rate of free-radical formation was smaller when cholesterol was present in the membrane than in membranes free of cholesterol. The α-tocopherol antioxidant effect in the membrane was also lower when cholesterol was present. We show here for the first time that 20-hydroxyecdysone has an antioxidant action in combination with α-tocopherol in membranes with cholesterol overload. Kinetic measurements were revealed the 20-hydroxyecdysone antioxidant effect on free radical reactions in membranes.
- 著者
- Tomoko AOKI Ikuko OTAKE Naohiro GOTOH Noriko NOGUCHI Shun WADA
- 出版者
- 公益社団法人 日本油化学会
- 雑誌
- Journal of Oleo Science (ISSN:13458957)
- 巻号頁・発行日
- vol.53, no.6, pp.285-294, 2004 (Released:2004-05-15)
- 参考文献数
- 39
- 被引用文献数
- 6 11
A new quantification method for triglyceride (TG) molecular species contained in fish oil was developed using high performance liquid chromatography (HPLC)-ultraviolet detector (UV) system. In this experiment, triacontyl silane column and a mixture of alcohol and acetonitrile were used for column and mobile phase, respectively. Fifteen kinds of TG molecular species exist in fish oil were collected and the calibration curves monitored at 210nm were acquired for each TG molecular species. Also, evaporative light scattering detector (ELSD), widely used for the detection of fish oil TG molecular species, was tandem jointed after UV to compare the calibration curves for each TG molecular species. As the results, the calibration curves by UV with isocratic elution system were linear lines, on the contrary, those by ELSD were not linear. The slope of each calibration curve by UV was not the same and there was a tendency that TG molecular species having big partition number indicates a small slope calibration curve. The HPLC-UV with gradient system was also examined, but a few of standard TGs did not provide linear calibration curve. Consequently, we concluded that isocratic HPLC-UV system would be an available method for the quantification of TG molecular species in fish oil.