- 著者
-
Momose Masaki
Itoh Yoshio
Umemoto Naoyuki
Nakayama Masayoshi
Ozeki Yoshihiro
- 出版者
- Japanese Society of Breeding
- 雑誌
- Breeding Science (ISSN:13447610)
- 巻号頁・発行日
- vol.63, no.4, pp.435-440, 2013
- 被引用文献数
-
13
A glutathione <i>S</i>-transferase-like gene, <i>DcGSTF2</i>, is responsible for carnation (<i>Dianthus caryophyllus</i> L.) flower color intensity. Two defective genes, <i>DcGSTF2mu</i> with a nonsense mutation and <i>DcGSTF2-dTac1</i> containing a transposable element <i>dTac1</i>, have been characterized in detail in this report. <i>dTac1</i> is an active element that produces reverted functional genes by excision of the element. A pale-pink cultivar 'Daisy' carries both defective genes, whereas a spontaneous deep-colored mutant 'Daisy-VPR' lost the element from <i>DcGSTF2-dTac1</i>. This finding confirmed that <i>dTac1</i> is active and that the resulting reverted gene, <i>DcGSTF2rev1</i>, missing the element is responsible for this color change. Crosses between the pale-colored cultivar '06-LA' and a deep-colored cultivar 'Spectrum' produced segregating progeny. Only the deep-colored progeny had <i>DcGSTF2rev2</i> derived from the 'Spectrum' parent, whereas progeny with pale-colored flowers had defective forms from both parents, <i>DcGSTF2mu</i> and <i>DcGSTF2-dTac1</i>. Thus, <i>DcGSTF2rev2</i> had functional activity and likely originated from excision of <i>dTac1</i> since there was a footprint sequence at the vacated site of the <i>dTac1</i> insertion. Characterizing the <i>DcGSTF2</i> genes in several cultivars revealed that the two functional genes, <i>DcGSTF2rev1</i> and <i>DcGSTF2rev2</i>, have been used for some time in carnation breeding with the latter in use for more than half a century.