著者

Momose Masaki Itoh Yoshio Umemoto Naoyuki Nakayama Masayoshi Ozeki Yoshihiro

出版者

Japanese Society of Breeding

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

vol.63, no.4, pp.435-440, 2013

被引用文献数

13

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A glutathione <i>S</i>-transferase-like gene, <i>DcGSTF2</i>, is responsible for carnation (<i>Dianthus caryophyllus</i> L.) flower color intensity. Two defective genes, <i>DcGSTF2mu</i> with a nonsense mutation and <i>DcGSTF2-dTac1</i> containing a transposable element <i>dTac1</i>, have been characterized in detail in this report. <i>dTac1</i> is an active element that produces reverted functional genes by excision of the element. A pale-pink cultivar 'Daisy' carries both defective genes, whereas a spontaneous deep-colored mutant 'Daisy-VPR' lost the element from <i>DcGSTF2-dTac1</i>. This finding confirmed that <i>dTac1</i> is active and that the resulting reverted gene, <i>DcGSTF2rev1</i>, missing the element is responsible for this color change. Crosses between the pale-colored cultivar '06-LA' and a deep-colored cultivar 'Spectrum' produced segregating progeny. Only the deep-colored progeny had <i>DcGSTF2rev2</i> derived from the 'Spectrum' parent, whereas progeny with pale-colored flowers had defective forms from both parents, <i>DcGSTF2mu</i> and <i>DcGSTF2-dTac1</i>. Thus, <i>DcGSTF2rev2</i> had functional activity and likely originated from excision of <i>dTac1</i> since there was a footprint sequence at the vacated site of the <i>dTac1</i> insertion. Characterizing the <i>DcGSTF2</i> genes in several cultivars revealed that the two functional genes, <i>DcGSTF2rev1</i> and <i>DcGSTF2rev2</i>, have been used for some time in carnation breeding with the latter in use for more than half a century.

著者

Yasumoto Shuhei Umemoto Naoyuki Lee Hyoung Jae Nakayasu Masaru Sawai Satoru Sakuma Tetsushi Yamamoto Takashi Mizutani Masaharu Saito Kazuki Muranaka Toshiya

出版者

日本植物細胞分子生物学会

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.36, no.3, pp.167-173, 2019

被引用文献数

32

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<p>Potato (<i>Solanum tuberosum</i>) is one of the most important crops in the world. However, it is generally difficult to breed a new variety of potato crops because they are highly heterozygous tetraploid. Steroidal glycoalkaloids (SGAs) such as α-solanine and α-chaconine found in potato are antinutritional specialized metabolites. Because of their toxicity following intake, controlling the SGA levels in potato varieties is critical in breeding programs. Recently, genome-editing technologies using artificial site-specific nucleases such as TALEN and CRISPR-Cas9 have been developed and used in plant sciences. In the present study, we developed a highly active Platinum TALEN expression vector construction system, and applied to reduce the SGA contents in potato. Using <i>Agrobacterium-</i>mediated transformation, we obtained three independent transgenic potatoes harboring the TALEN expression cassette targeting SSR2 gene, which encodes a key enzyme for SGA biosynthesis. Sequencing analysis of the target sequence indicated that all the transformants could be <i>SSR2</i>-knockout mutants. Reduced SGA phenotype in the mutants was confirmed by metabolic analysis using LC-MS. In vitro grown <i>SSR2</i>-knockout mutants exhibited no differences in morphological phenotype or yields when compared with control plants, indicating that the genome editing of SGA biosynthetic genes such as <i>SSR2</i> could be a suitable strategy for controlling the levels of toxic metabolites in potato. Our simple and powerful plant genome-editing system, developed in the present study, provides an important step for future study in plant science.</p>