著者

Ryoutarou OISHI Tatsumi MIZUTA Kenji SUEYOSHI Tatsuro ENDO Hideaki HISAMOTO

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.36, no.2, pp.143-145, 2020-02-10 (Released:2020-02-10)

参考文献数

30

被引用文献数

9

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Herein, we describe the development of a novel material, “enzyme-responsive fluorescent ionic liquid”, which enabled a highly sensitive detection of alkaline phosphatase (ALP). We prepared a plasticized poly(vinyl chloride) (PVC) membrane using this new material as a plasticizer and quantified ALP in aqueous solutions. Preliminary results suggested that the PVC membrane responded to ALP at an interface between the membrane and the sample solution with anion extraction to maintain electroneutrality in the membrane phase. The developed PVC membrane showed an approximately six-times higher sensitivity than the conventional membrane, thereby demonstrating highly sensitive ALP detection. These results suggested the potential applicability of the proposed membrane for highly sensitive protein detection by using ALP-labeled antibodies.