著者
Yuki IWAHANA Atsushi OHBUCHI Yuya KOIKE Masaru KITANO Toshihiro NAKAMURA
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.29, no.1, pp.61-66, 2013-01-10 (Released:2013-01-10)
参考文献数
26
被引用文献数
5 19

Radioactive nuclides in the incinerator ashes of municipal solid wastes were determined by γ-ray spectrometry before and after the accident at the Fukushima nuclear power plant (March 11, 2011). Incinerator ash samples were collected in northern Kyushu, Japan, which is located approximately 1200 km west-southwest (WSW) of the Fukushima nuclear power plant, from April 2006 to March 2007 and from March 2011 to October 2011. 40K, 137Cs, 208Tl, 212Pb, 214Pb, 212Bi, 214Bi, and 228Ac were identified in the ashes before the accident (∼February 2011) and 134Cs was identified along with these eight nuclides in the ashes after the accident (March 2011∼). A sequential extraction procedure based on a modified Tessier method with added water extraction was used for 1st fly ash sampled in August 2011 because the highest activity concentrations of 134Cs and 137Cs were observed for this sample. The speciation of radioactive nuclides in the fly ash was achieved by γ-ray spectrometry and powder X-ray diffractometry for the extraction residues. Little variation was observed in the distribution of the chemical forms of 134Cs and 137Cs in 1st fly ash of municipal solid waste; one half of 134Cs existed as water soluble salts and the other half as carbonate compounds, whereas 75% of 137Cs existed as water soluble salts with the remainder as carbonates(10%) and sulfides (15%). These results show that 88% of the total radioactive Cs existed in water soluble and ion extractive forms and might be at risk for elution and diffusion with rain and wind.
著者
Akihiko ISHIDA Mitsutaka FUJII Takehiro FUJIMOTO Shunsuke SASAKI Ichiro YANAGISAWA Hirofumi TANI Manabu TOKESHI
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.31, no.11, pp.1163-1169, 2015-11-10 (Released:2015-11-10)
参考文献数
33
被引用文献数
25 37

A compact and lightweight liquid chromatography system is presented with overall dimensions of 26 cm width × 18 cm length × 21 cm height and weight of 2 kg. This system comprises a battery-operated compact electroosmotic pump, a manual injector, a microfluidic chip device containing a packed column and an electrochemical detector, and a USB bus-powered potentiostat. The pumping system was designed for microfluidic-based reversed-phase liquid chromatography in which an electroosmotically generated water stream pushes the mobile phase via a diaphragm for the output. The flow rate ranged from 0 to 10 μL/min and had a high degree of precision. The pumping system operated continuously for over 24 h with dry batteries. The column formed in the microfluidic device was packed with 3-μm ODS particles with a length of 30 mm and a diameter of 0.8 mm. The results presented herein demonstrate the performance of the pumping system and the column using alkylphenols, catecholamine, catechin, and amino acids.
著者
Hiroshi IWANAGA Hirohiko TSUZUKI Yoichiro KAMIYAMA Hiroshi UEDA
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.25, no.9, pp.1101-1106, 2009-09-10 (Released:2009-09-10)
参考文献数
13
被引用文献数
1 2 3

With a specific and strong molecular-recognition capability cultivated in humoral acquired immunity, an antibody has been extensively utilized in various applications, such as diagnostics and therapy. However, so far most of its uses have been limited to be in the liquid phase. In view of its potential uses, such as a gas-phase biosensor or a high-performance air filter, we have tried to verify a previously undescribed binding reaction between protein antigens and corresponding antibodies immobilized on a solid surface by using fluorescence resonance energy transfer between the two. Our data showed that the antibody on a solid surface specifically reacted with a protein antigen supplied from the gas phase under the normal ambient condition. Also discovered was that the reaction occurred even faster than that in the liquid phase under several assay conditions.
著者
Nobutoshi OTA Genki N. KANDA Hiroyuki MORIGUCHI Yusufu AISHAN Yigang SHEN Rikuhiro G. YAMADA Hiroki R. UEDA Yo TANAKA
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.35, no.10, pp.1141-1147, 2019-10-10 (Released:2019-10-10)
参考文献数
24
被引用文献数
5

Microfluidic devices are important platforms to culture and observe biological tissues. Compared with conventional setups, microfluidic devices have advantages in perfusion, including an enhanced delivery of nutrients and gases to tissues. However, explanted tissues can maintain their functions for only hours to days in microfluidic devices, although their observations are desired for weeks. The suprachiasmatic nucleus (SCN) is a brain region composed of heterogeneous cells to control the biological clock system through synchronizing individual cells in this region. The synchronized and complicated cell–cell interactions of SCN cells are difficult to reproduce from seeded cells. Thus, the viability of explanted SCN contributes to the study of SCN functions. In this paper, we propose a new perfusion platform combining a PDMS microfluidic device with a porous membrane to culture an explanted SCN for 25 days. We expect that this platform will provide a universal interface for microfluidic manipulation of tissue explants.
著者
Tomoko OHTA Yasunori MAHARA Takumi KUBOTA Toshifumi IGARASHI
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.29, no.10, pp.941-947, 2013-10-10 (Released:2013-10-10)
参考文献数
30
被引用文献数
1 4

We measured 134Cs and 137Cs in the surface soil of the Kanto loam in the eastern Tokyo metropolitan area and the Nishiyama loam in Nagasaki, Japan. The observed 137Cs deposition in the Kanto loam from the Fukushima nuclear power plant (NPP) accident ranged from 4.0 to 77 kBq m−2, which corresponds to 0.3 – 5 times of that in the Nishiyama loam. The 137Cs retardation factor in the Kanto loam obtained seven months after the Fukusima NPP accident and in the Nishiyama loam after 36 and 38 years from the detonation of the Pu atomic bomb (A-bomb) ranged from 180 to 260 and 2000 to 10000, respectively. This difference in the retardation factors is attributed to an aging effect that corresponds to seven months and 36 to 38 years after the deposition of 137Cs occurred on the soil minerals.
著者
Seitaro OHTSU Masamitsu YAMAGUCHI Hisashi NISHIWAKI Eiichiro FUKUSAKI Shuichi SHIMMA
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.34, no.9, pp.991-996, 2018-09-10 (Released:2018-09-10)
参考文献数
19
被引用文献数
12

Imidacloprid is widely used for exterminating harmful insects; however, information regarding its distribution in insects is limited. Herein, we developed a visualization method for imidacloprid in Drosophila melanogaster, by using matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). IMS requires sample cryosections; however, certain challenges prevail in retaining fly morphology in sections owing to their small size and heterogeneous components. Therefore, the section preparation method was optimized first, followed by imidacloprid distribution visualized using MALDI-IMS. Using 10% gelatin as an embedding material and 70% ethanol for pretreatment, the gaps between embedding material and D. melanogaster body surface were reduced. The tight adhesion between embedding media and D. melanogaster retains fly morphology in sections. Furthermore, the imidacloprid standard was analyzed separately via MALDI and electrospray ionization (ESI), and imidacloprid was converted to guanidine-imidacloprid via laser irradiation. Consequently, the imidacloprid distribution in D. melanogaster was successfully visualized using guanidine-imidacloprid as the target peak.
著者
Takeshi OHNO Atsuko SHINOHARA Ichitaro KOHGE Momoko CHIBA Takafumi HIRATA
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.20, no.4, pp.617-621, 2004 (Released:2004-10-08)
参考文献数
18
被引用文献数
33 54

Precise 56Fe/54Fe and 57Fe/54Fe isotopic ratios on human red blood cell (RBC) samples have been measured using multiple collector-ICP-mass spectrometry (MC-ICPMS). The mass spectrometric interferences on Fe isotopes (e.g., 56ArO+ and 57ArOH+) were successfully minimized by a dry plasma condition achieved by a desolvating nebulizer sample-introduction technique. In order to eliminate possible variations in the measured isotopic ratios due to non-mass spectrometric interferences, Fe was separated from remaining organic compounds and major co-existing elements using an ion chromatographic technique. The resulting precisions of the 56Fe/54Fe and 57Fe/54Fe ratio measurements were 0.12‰ and 0.20‰, respectively, which were high enough to detect the isotopic variation of Fe in nature. For an interlaboratory comparison, all of the Fe isotopic ratio data were normalized by the ratios for the IRMM-014 international isotopic standard. A series of 12 RBC samples were collected from one person through monthly-based sampling over a period of one year. These were analyzed to test possible seasonal changes in the 56Fe/54Fe and 57Fe/54Fe ratios. Moreover, in order to test possible variations in the 56Fe/54Fe and 57Fe/54Fe ratios among different people, RBC samples were collected from five volunteers (four males and one female). The 56Fe/54Fe and 57Fe/54Fe ratios for a series of 12 RBC samples collected over a one-year period show 3.06‰ and 4.51‰ lower than the values of IRMM-014, and no significant seasonal change could be found in the ratios. The lack in seasonal changes in the Fe isotopic ratios could be explained by a small contribution of the daily net-intake of Fe (1 - 2 mg/day) onto the total amount of Fe in the human body (2 - 4 g). The 56Fe/54Fe and 57Fe/54Fe ratios for RBC samples collected from four male samples did not vary measurably, whereas the Fe isotopic ratios for a female RBC were 0.3‰/amu heavier than the mean value of four male samples. This difference in Fe isotopes among the individuals can be the result of a difference in uptake efficiency of the Fe through a dietary process from the digestive tract. The data obtained here demonstrate that the isotopic ratios of trace metals can provide new information about metabolic efficiencies of the metallic elements.
著者
Misaki HATA Masato SUZUKI Tomoyuki YASUKAWA
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.37, no.6, pp.803-806, 2021-06-10 (Released:2021-06-10)
参考文献数
32
被引用文献数
4

We proposed selective manipulation techniques for retrieving and retaining target cells arrayed in microwells based on dielectrophoresis (DEP). The upper substrate with microband electrodes was mounted on the lower substrate with microwells based on the same design of microband electrodes by 90 degree relative to the lower substrate. A repulsive force of negative dielectrophoresis (n-DEP) was employed to retrieve the target cells from the microwell array selectively. Furthermore, the target cells were retained in the microwells after other cells were removed by n-DEP. Thus, the system described in this study could make it possible to retrieve and recover single target cells from a microwell array after determining the function of cells trapped in each microwell.
著者
Yuki HIRAKAWA Tomomi YAMASAKI Ayako HARADA Seiji IWASA Hiroshi NARITA Shiro MIYAKE
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.34, no.5, pp.533-539, 2018-05-10 (Released:2018-05-10)
参考文献数
30
被引用文献数
17

A simultaneous immunosensor based on surface plasmon resonance (SPR) was developed for determination of 3 pesticides —boscalid, clothianidin and nitenpyram— instead of the direct competitive enzyme-linked immunosorbent assays (dcELISAs) widely used as individual determination methods. Carboxy groups that introduced compounds to their pesticides were designed, and conjugates of them and bovine serum albumin were immobilized onto separate channels of the same sensor chip. When a mixture of 3 monoclonal antibodies reacted to each pesticide, and 3 pesticides were injected into the SPR immunosensor, each channel showed specific reactivity at 15 – 93 ng mL−1 for boscalid, 6.7 – 27 ng mL−1 for clothianidin, and 7.3 – 62 ng mL−1 for nitenpyram. Recovery tests using vegetables spiked with a mixture of 3 pesticides showed good results: 75 – 90%, 88 – 104%, and 72 – 105%, respectively, with a high correlation to results of the dcELISAs. The SPR immunosensor would be useful for the determination of pesticide residues in vegetables.
著者
Suchada CHUANUWATANAKUL Orawon CHAILAPAKUL Shoji MOTOMIZU
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.24, no.4, pp.493-498, 2008 (Released:2008-04-10)
参考文献数
40
被引用文献数
36 46

The electrochemical properties of chloramphenicol at a boron-doped diamond thin-film (BDD) electrode were studied using cyclic voltammetry. The highest current response of chloramphenicol was obtained with phosphate buffer, pH 6 (0.1 M) in 1% ethanol. The relationship between the concentration of chloramphenicol and the current response was linear over the range of 0.1 - 10 mM (R2 = 0.9990). The amount of chloramphenicol was analyzed by flow-injection analysis. A thin-layer flow cell equipped with a BDD electrode was used as an amperometric detector, and experiments were carried out at -0.7 V (vs. Ag/AgCl). The linear relationship between the current response and the concentration of chloramphenicol in the range of 0.1 - 50 µM (R2 = 0.9948) and the limit of detection of 0.03 µM (S/N = 3) were obtained. This method has been successfully applied to the determination of chloramphenicol in sterile eye drops and milk sample by the standard addition method. The average recoveries of chloramphenicol in eye drops were 98.0%, and the average recoveries of chloramphenicol from spiked milk were 93.9 - 103%.
著者
Yingying ZHANG Luhui WANG Yafei DONG
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.35, no.2, pp.181-187, 2019-02-10 (Released:2019-02-10)
参考文献数
32
被引用文献数
3 4

Due to structual polymorphism, excellent binding activity and functional significances in biological regulation, G-quadruplex has become the focus of research as an innovated module for analytical chemistry and biomedicine. Meanwhile, in the biosensor fields, new nanomaterial graphene oxide (GO) has also received extensive attention due to its brilliant physical and chemical properties. Herein, we propose a non-label and enzyme-free logic operation platform based on G-quadruplex structure and GO instead of any expensive modification. Taking advantage of the quenching ability of GO to AgNCs and the fluorescence enhancement of NMM (N-methylmesoporphyrin IX) mediated by the split G-quadruplex, a series of binary logic gates (AND, OR, INHIBIT, XOR) have been constructed and verified by biological experiments. Subsequently, two combinatorial logic gates were successfully realized conceptually on the basis of the same BGG platform, including half adder and half subtractor. Taken together, such a universal platform has great potential in applications, such as biocomputing, bio-imaging and disease diagnosis, which cultivate a new view for future biological research.
著者
Momona HORIGUCHI Shinsuke KUNIMURA
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.36, no.11, pp.1311-1315, 2020-11-10 (Released:2020-11-10)
参考文献数
14
被引用文献数
4

This paper describes a method for obtaining information that can contribute to individual identification from a single hair colored with a hair dye product using a combination of surface-enhanced Raman scattering (SERS) and X-ray fluorescence (XRF) analyses. SERS and XRF spectra of single hog hairs colored with several commercially available hair dye products were measured. SERS spectral patterns tended to be different depending on the hair dye products used for hair coloring. However, SERS spectral patterns of single hog hairs colored with different hair dye products that would produce similar types of dyes were similar. By performing XRF analysis, characteristic metallic elements originating from some hair dye products were detected. Therefore, XRF can contribute to identifying the difference among colored hairs that cannot be identified only by SERS. SERS and XRF analyses of a single shed hair can contribute to individual identification used in forensic science.
著者
Masanari NAGASAKA Hayato YUZAWA Nobuhiro KOSUGI
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.36, no.1, pp.95-105, 2020-01-10 (Released:2020-01-10)
参考文献数
100
被引用文献数
16

Soft X-ray absorption spectroscopy (XAS) involving excitation processes of a core electron to unoccupied states is an effective method to study local structures around excited C, N, and O atoms in liquid samples. Since soft X-rays are strongly absorbed by air and liquid itself, we have developed transmission-type liquid flow cells, where the absorbance of liquid samples can be easily reduced and optimized by controlling the liquid thickness. By using the transmission-mode XAS techniques, we have investigated local structures of several liquid samples such as concentration dependence of aqueous pyridine solutions and unexpected temperature-dependent structural changes in liquid benzene from the precise energy shift measurements in XAS spectra with the help of molecular dynamics simulation and inner-shell calculations. These XAS techniques are also applied to in situ/operando observation of chemical processes in solutions such as catalytic and electrochemical reactions.