- 著者
- SHIMOKAWA Tomoko YOSHIDA Shigeki TAKEUCHI Toshio MURATA Katsumi ISHII Tadashi KUSAKABE Isao
- 出版者
- 社団法人日本農芸化学会
- 雑誌
- Bioscience, biotechnology, and biochemistry (ISSN:09168451)
- 巻号頁・発行日
- vol.60, no.9, pp.1532-1534, 1996-09-23
- 被引用文献数
- 4 36
The objective of this study was to prepare two series of authentic oligo-guluronic acids from sodium alginate. Oligo-guluronic acids (DP=1-9) were prepared from an acid hydrolysate of poly-guluronic acid by successive chromatographies of Bio-Gel P-6 and Q Sepharose Fast Flow. Oligo-guluronic acids having 4-deoxy-L-erythro-hex-4-enopyranosyluronic acid residues at the non-reducing end (DP=2-7) were prepared from the enzymatic degradation products of the poly-guluronic acid in the same manner. Each of the isolated oligo-guluronic acids gave a single band on fluorophore-assisted carbohydrate electrophoresis. These results suggest that successive chromatographies used in this study are well suited for the preparation of alginate-derived oligouronic acids.