著者

Seitaro Ohkuma Shi-Hu Chen Masashi Katsura Da-Zhi Chen Kinya Kuriyama

出版者

The Japanese Pharmacological Society

雑誌

The Japanese Journal of Pharmacology (ISSN:00215198)

巻号頁・発行日

vol.64, no.2, pp.125-128, 1994 (Released:2006-04-10)

参考文献数

19

被引用文献数

32 34

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The effect of muscimol on N-methyl-D-aspartate (NMDA)-induced injury of primary cultured cerebral cortical neurons was examined. NMDA induced a dose-dependent leakage of LDH activity, which was significantly inhibited by (±)-5-methyl-10, 11-dihydro-5H-dibenzo-[a, d]cyclopentan-5, 10-imine (MK-801). Muscimol significantly reduced the NMDA-induced increase of lactic dehydrogenase (LDH) leakage, and bicuculline abolished this protective effect of muscimol. Similarly, muscimol reduced the NMDA-induced increase in trypan blue staining of the cells, and bicuculline suppressed this inhibitory action of muscimol. These results suggest that GABAA-receptor stimulation exerts a protective action against the neuronal injury induced by NMDA-receptor activation.