- 著者
- Gaoshuang Hu Dan Su Tianqi Wu Tao Zhao Longhua Xu Shan Gao Jianxiong Hao
- 出版者
- Japanese Society for Food Science and Technology
- 雑誌
- Food Science and Technology Research (ISSN:13446606)
- 巻号頁・発行日
- pp.FSTR-D-22-00115, (Released:2022-09-30)
- 被引用文献数
- 1
Portable glucose meter (PGM) has played vital roles in the field of medicine, with advantages of accurate and quantitative results, portability, easy operation, and low cost. Recently, more and more researchers have extended the application of PGM in detecting foods contaminations. In this paper, a review of the application of PGM based on target recognition elements, such as antibody–antigen reaction, molecularly imprinted polymer, nucleic acid hybridization, enzyme recognition, and click chemistry, and amplification strategies, in food safety and contaminant analysis are described in detail. The future development of the application of glucose meters in food safety supervision is also discussed.
- 著者
- Qing-Shan GAO Mei-Fu XUAN Zhao-Bo LUO Hyo-Jin PAEK Jin-Dan KANG Xi-Jun YIN
- 出版者
- Japanese Association for Laboratory Animal Science
- 雑誌
- Experimental Animals (ISSN:13411357)
- 巻号頁・発行日
- pp.19-0018, (Released:2019-07-15)
- 被引用文献数
- 3
The nuclear receptor corepressor Hairless (HR) interacts with nuclear receptors and controls expression of specific target genes involved in hair morphogenesis and hair follicle cycling. Patients with HR gene mutations exhibit atrichia, and in rare cases, immunodeficiency. Pigs with HR gene mutations may provide a useful model for developing therapeutic strategies because pigs are highly similar to humans in terms of anatomy, genetics, and physiology. The present study aimed to knockout the HR gene in pigs using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated-9 system and to investigate the molecular and structural alterations in the skin and thymus. We introduced a biallelic mutation into the HR gene in porcine fetal fibroblasts and generated nine piglets via somatic cell nuclear transfer. These piglets exhibited a lack of hair on the eyelids, abnormalities in the thymus and peripheral blood, and altered expression of several signaling factors regulated by HR. Our results indicate that introduction of the biallelic mutation successfully knocked out the HR gene, resulting in several molecular and structural changes in the skin and thymus. These pigs will provide a useful model for studying human hair disorders associated with HR gene mutations and the underlying molecular mechanisms.