著者
Kei Fujiwara Miho Yanagisawa Shin-ichiro M. Nomura
出版者
日本生物物理学会
雑誌
BIOPHYSICS (ISSN:13492942)
巻号頁・発行日
vol.10, pp.43-48, 2014 (Released:2014-08-07)
参考文献数
38
被引用文献数
1 15

Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomacromolecules in artificial cells. In this review, we introduce our recent progress to reconstitute intracellular environments in vitro and in artificial cells.