- 著者
- Yuki HIRAKAWA Tomomi YAMASAKI Ayako HARADA Seiji IWASA Hiroshi NARITA Shiro MIYAKE
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.34, no.5, pp.533-539, 2018-05-10 (Released:2018-05-10)
- 参考文献数
- 30
- 被引用文献数
- 18
A simultaneous immunosensor based on surface plasmon resonance (SPR) was developed for determination of 3 pesticides —boscalid, clothianidin and nitenpyram— instead of the direct competitive enzyme-linked immunosorbent assays (dcELISAs) widely used as individual determination methods. Carboxy groups that introduced compounds to their pesticides were designed, and conjugates of them and bovine serum albumin were immobilized onto separate channels of the same sensor chip. When a mixture of 3 monoclonal antibodies reacted to each pesticide, and 3 pesticides were injected into the SPR immunosensor, each channel showed specific reactivity at 15 – 93 ng mL−1 for boscalid, 6.7 – 27 ng mL−1 for clothianidin, and 7.3 – 62 ng mL−1 for nitenpyram. Recovery tests using vegetables spiked with a mixture of 3 pesticides showed good results: 75 – 90%, 88 – 104%, and 72 – 105%, respectively, with a high correlation to results of the dcELISAs. The SPR immunosensor would be useful for the determination of pesticide residues in vegetables.