著者

Sota Nishimoto Katsuyuki Hamasaki Shigeki Dan

出版者

Carcinological Society of Japan

雑誌

Crustacean Research (ISSN:02873478)

巻号頁・発行日

vol.52, pp.23-30, 2023-02-18 (Released:2023-02-18)

参考文献数

23

被引用文献数

2

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We studied the artificial incubation method of red swamp crayfish Procambarus clarkii embryos using cell culture plates. In the first experiment, cleavage stage embryos or egg-nauplius stage embryos were individually cultured at 20°C in the well of cell culture plates with different water volumes per well [12 well (4 ml), 24 well (2 ml), 48 well (1 ml) and 96 well (0.25 ml)]. In the second experiment, egg-nauplius stage embryos were cultured using 12-well plates at 15, 20 and 25°C. Almost all cleavage stage embryos died without hatching. Egg-nauplius stage embryos survived to hatch. In the 96-well plates, the embryo hatched, but all first instar juveniles died soon. In other plates, the mortality of the first instar juveniles tended to increase with decreasing culture water volume; particularly, high mortality was recorded in the 48-well plates. Hatching and moulting rates were high at 15°C and 20°C, but approximately half of the first instar juveniles died at 25°C. We concluded that a 12-well cell culture plate (4 ml per well) is suitable for incubating P. clarkii embryos to hatch and the first instar juveniles to moult to the second instar at 15–20°C.

著者

Sota Nishimoto Katsuyuki Hamasaki Shigeki Dan

出版者

Carcinological Society of Japan

雑誌

Crustacean Research (ISSN:02873478)

巻号頁・発行日

vol.51, pp.103-110, 2022-09-17 (Released:2022-09-17)

参考文献数

32

被引用文献数

3

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The red swamp crayfish Procambarus clarkii is an invasive alien species worldwide but it was commercially exploited. Laboratory studies have been conducted to examine the reproductive aspects of this species, pairing males and females for 1–5 days, as a basis for developing the control measures for invasive populations and improving aquaculture technologies. However, the effect of pairing periods on the mating and spawning success is largely unknown, and the present study therefore aimed to elucidate the effect of different periods (one, five and 10 days) in this species. The pairs were videorecorded for each entire pairing period, and the length of pairing did not significantly affect copulation or spawning. Copulation was prolific on the first day and its intensity was similar among the test groups, indicating that a one-day pairing period is sufficient for successful mating in P. clarkii under laboratory conditions.