著者
Tomoki Nishimura Takeshi Cho Andrew M. Kelley Magdalena E. Powell John S. Fossey Steven D. Bull Tony D. James Hiroyasu Masunaga Isamu Akiba Kazuo Sakurai
出版者
The Chemical Society of Japan
雑誌
Bulletin of the Chemical Society of Japan (ISSN:00092673)
巻号頁・発行日
pp.1008170144, (Released:2010-08-21)
参考文献数
42
被引用文献数
10

Synthetic DNA-transfection reagents can overcome safety issues raised by use of viral DNA vectors. One of these candidates is a cationic lipid that can form a supramolecular complex with DNA. We have been working in a series of aromatic diamine lipids with different tail length from C6 to C18: [N-(3,5-dialkylbenzyl)ethane-1,2-diamine, denoted DA] as such a lipid. The present paper describes the synthesis and the fundamental properties of DA. SAXS from DA solution showed bilayer vesicle formation, while it showed lamellar formation after the complexation with DNA. When we measured the N/P ratio (molar ratio of the amine groups (N) in DA to phosphate groups (P) in the DNA) dependence of SAXS, the lamellar peak (spacing = 5.0 nm) increased proportionally to the added DNA concentration at N/P ≥ 8. On the other hand, in the range of N/P < 8, the spacing was increased to 5.5 nm and the area decreased as DNA increased. These different features between N/P ≥ 8 and N/P < 8 suggest that the lamellar supramolecular structures differ according to the composition.