著者

Yoshiya Maegawa Shinichi Mochizuki Noriko Miyamoto Yusuke Sanada Kazuo Sakurai

出版者

FCCA(Forum: Carbohydrates Coming of Age)

雑誌

Trends in Glycoscience and Glycotechnology (ISSN:09157352)

巻号頁・発行日

vol.27, no.153, pp.13-29, 2015-01-25 (Released:2015-01-23)

参考文献数

64

被引用文献数

1

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β-1,3-D-グルカンの一種であるシゾフィラン(SPG)はホモ配列のオリゴデオキシヌクレオチド(ODN)と水素結合や疎水性相互作用によってODN/SPG複合体を形成する。また、マクロファージや樹状細胞などの抗原提示細胞上には、β-1,3-D-グルカンの受容体であるデクチン-1が発現している。そのため、この複合体を用いることで、アンチセンスODN(AS-ODN)や非メチル化CpG配列を持ったODN(CpG-ODN)を抗原提示細胞に特異的に送達することが可能になると考えられる。実際、AS-ODN/SPG複合体をリポ多糖誘導型マウス肝炎モデルに投与したとき、炎症を抑えることができた。また、カニクイザルにCpG-ODN/SPG複合体をインフルエンザワクチンのアジュバントとして投与したとき、高い抗体価を促すことができた。以上より、SPGは特に抗原提示細胞を標的にした薬物送達システムのキャリアとして有用であると考えられる。

著者

Tomoki Nishimura Takeshi Cho Andrew M. Kelley Magdalena E. Powell John S. Fossey Steven D. Bull Tony D. James Hiroyasu Masunaga Isamu Akiba Kazuo Sakurai

出版者

The Chemical Society of Japan

雑誌

Bulletin of the Chemical Society of Japan (ISSN:00092673)

巻号頁・発行日

pp.1008170144, (Released:2010-08-21)

参考文献数

42

被引用文献数

10

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Synthetic DNA-transfection reagents can overcome safety issues raised by use of viral DNA vectors. One of these candidates is a cationic lipid that can form a supramolecular complex with DNA. We have been working in a series of aromatic diamine lipids with different tail length from C6 to C18: [N-(3,5-dialkylbenzyl)ethane-1,2-diamine, denoted DA] as such a lipid. The present paper describes the synthesis and the fundamental properties of DA. SAXS from DA solution showed bilayer vesicle formation, while it showed lamellar formation after the complexation with DNA. When we measured the N/P ratio (molar ratio of the amine groups (N) in DA to phosphate groups (P) in the DNA) dependence of SAXS, the lamellar peak (spacing = 5.0 nm) increased proportionally to the added DNA concentration at N/P ≥ 8. On the other hand, in the range of N/P < 8, the spacing was increased to 5.5 nm and the area decreased as DNA increased. These different features between N/P ≥ 8 and N/P < 8 suggest that the lamellar supramolecular structures differ according to the composition.

著者

HISAYUKI KATO TADAO HATTORI MAKOTO URANO KAZUO SAKURAI TATSUYOSHI OKADA TAKEHIRO YUI KENSEI NAITO

出版者

JAPAN SOCIETY FOR HEAD AND NECK SURGERY

雑誌

頭頸部外科 (ISSN:1349581X)

巻号頁・発行日

vol.20, no.2, pp.185-190, 2010-10-30 (Released:2010-12-10)

参考文献数

16

被引用文献数

3 3

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We report an unusual case of ectopic cervical thymoma. A 35-year-old female patient presented with an anterior neck mass that developed over a five-month duration. The patient underwent fine-needle aspiration cytology (FNAC) twice, but the tumor type could not be diagnosed. The surgically removed tumor was white in color and had a smooth surface. According to the postoperative pathological examination, the tumor was diagnosed as a Stage I ectopic cervical thymoma and Type B1 according to the Masaoka staging system and the World Health Organization (WHO) histological staging system, respectively. The patient was in good overall health and remained without tumor recurrence at the time of the 10-month follow-up. Because it was difficult to make a preoperative definite diagnosis by FNAC for the thymoma, we constructed a cell block from the existing cytological specimen. Immunohistological staining using the cell-block method clearly demonstrated the biphasic cellular population pattern that is a characteristic of thymoma. Therefore, this method may be useful as a preoperative differential diagnostic modality for cervical thymomas.