著者
Kazusa Nishiyama Atsushi Yamada Miki Takahashi Tomoharu Takeuchi Ken-ichi Kasai Susumu Kobayashi Hideaki Natsugari Hideyo Takahashi
出版者
The Pharmaceutical Society of Japan
雑誌
Chemical and Pharmaceutical Bulletin (ISSN:00092363)
巻号頁・発行日
vol.58, no.4, pp.495-500, 2010-04-01 (Released:2010-04-10)
参考文献数
29
被引用文献数
15 17

To search for the endogenous glyco-epitope in Caenorhabditis elegans, we synthesized labeled Galβ1-3Fuc and Galβ1-4Fuc and examined their binding affinity for C. elegans galectin LEC-6 using frontal affinity chromatography analysis. We developed a new strategy for synthesizing the labeled saccharides, in which the labeling unit, the 2-aminopyridine moiety, is coupled with a spacer unit derived from D-mannitol. Our results indicate that Galβ1-4Fuc is the endogenous glyco-epitope present in C. elegans N-glycans.