著者

辻 忠夫 Tadao Tsuji

出版者

同志社大学経済学会

雑誌

經濟學論叢 = Keizaigaku-Ronso (The Doshisha University economic review) (ISSN:03873021)

巻号頁・発行日

vol.45, no.4, pp.1-36, 1994-10-31

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論説

著者

Yutaka SUZUKI Yoichi MIZUTANI Tadao TSUJI Naoto OHTANI Kazufumi TAKANO Mitsuru HARUKI Masaaki MORIKAWA Shigenori KANAYA

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.69, no.2, pp.364-373, 2005 (Released:2005-02-23)

参考文献数

39

被引用文献数

19

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The gene encoding alkaline phosphatase from the psychrotrophic bacterium Shewanella sp. SIB1 was cloned, sequenced, and overexpressed in Escherichia coli. The recombinant protein was purified and its enzymatic properties were compared with those of E. coli alkaline phosphatase (APase), which shows an amino acid sequence identity of 37%. The optimum temperature of SIB1 APase was 50 °C, lower than that of E. coli APase by 30 °C. The specific activity of SIB1 APase at 50 °C was 3.1 fold higher than that of E. coli APase at 80 °C. SIB1 APase lost activity with a half-life of 3.9 min at 70 °C, whereas E. coli APase lost activity with a half-life of >6 h even at 80 °C. Thus SIB1 APase is well adapted to low temperatures. Comparison of the amino acid sequences of SIB1 and E. coli APases suggests that decreases in electrostatic interactions and number of disulfide bonds are responsible for the cold-adaptation of SIB1 APase.