著者

Kiyotaka MORI Takashi OKADA Kazuya OGURI Yoshitake NISHI

出版者

Society of Advanced Science

雑誌

Journal of Advanced Science (ISSN:09155651)

巻号頁・発行日

vol.9, no.1-2, pp.49-54, 1997-06-30 (Released:2010-02-25)

参考文献数

7

被引用文献数

1 1

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Effects of car coating treatment on resistance to environmental corrosion and on cracks formed by collision were studied by microscopical, spectroscopical and mechanical methods. Treated and untreated samples aged for three years were used to examine the surface brightness, the water repellency and the crack appearance. From the results, it was verified that this excellent coating material mainly consists of silicone polymer and the treated car-body surface has resistance to corrosion and superelasticity.

著者

Shin SHIMADA Takahiro YOSHIZAWA Yuki TAKAHASHI Yuko NITAHARA-KASAHARA Takashi OKADA Yoshihiro NOMURA Hitoki YAMANAKA Tomoki KOSHO Kiyoshi MATSUMOTO

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.69, no.4, pp.407-413, 2020 (Released:2020-11-12)

参考文献数

19

被引用文献数

2 3

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Ehlers–Danlos syndromes (EDSs) are heterogeneous group of heritable connective tissue disorders characterized by joint and skin hyperextensibility as well as fragility of various organs. Recently, we described a new type of EDS, musculocontractual EDS (mcEDS-CHST14), caused by pathogenic variants of the carbohydrate sulfotransferase 14 (CHST14) gene mutation. B6;129S5-Chst14tm1Lex/Mmucd (B6;129-Chst14 KO) mice are expected to be an animal model of mcEDS-CHST14. However, >90% of B6;129-Chst14 KO homozygous (B6;129-Chst14−/−) mice show perinatal lethality. Therefore, improvement of the birth rate of Chst14−/− mice is needed to clarify the pathophysiology of mcEDS-CHST14 using this animal model. Some B6;129-Chst14−/− embryos had survived at embryonic day 18.5 in utero, suggesting that problems with delivery and/or childcare may cause perinatal lethality. However, in vitro fertilization and egg transfer did not improve the birth rate of the mice. A recent report showed that backcrossing to C57BL/6 strain induces perinatal death of all Chst14−/− mice, suggesting that genetic background influences the birthrate of these mice. In the present study, we performed backcrossing of B6;129-Chst14 KO mice to a BALB/c strain, an inbred strain that shows lower risks of litter loss than C57BL/6 strain. Upon backcrossing 1 to 12 times, the birth rate of Chst14−/− mice was improved with a birth rate of 6.12–18.64%. These results suggest that the genetic background influences the birth rate of Chst14−/− mice. BALB/c congenic Chst14−/− (BALB.Chst14−/−) mice may facilitate investigation of mcEDS-CHST14. Furthermore, backcrossing to an appropriate strain may contribute to optimizing animal experiments.

著者

岡田 孝 Takashi Okada

雑誌

情報科学研究 (ISSN:09120939)

巻号頁・発行日

no.24, pp.3-7, 2010-03-19