著者

Takumi TAKANO Chiaki AOYAMA Yoshiro TERASAKI Kenichi SUZUKI Aki ANDO Yanting SONG Makoto TSUNODA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.37, no.12, pp.1811-1814, 2021-12-10 (Released:2021-12-10)

参考文献数

15

被引用文献数

1

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Therapeutic oligonucleotides have recently been approved in the United States, the EU, and Japan. Hence, the analysis of oligonucleotides is an important topic in drug development. Liquid chromatographic techniques are commonly used for purity verification and the determination of oligonucleotides. In ion-pair reversed-phase separation, several parameters, such as the pore size of the stationary phase, mobile phase additives, and column temperature, were investigated using three types of oligonucleotides (18, 19, and 20 mer). All of the investigated parameters could influence the separation, and they are expected to be useful for optimizing oligonucleotide separation.