著者

Tomohiro Yako Yoshiki Kuse Shinsuke Nakamura Masamitsu Shimazawa Takashi Motomura Hideaki Hara

出版者

The Illuminating Engineering Institute of Japan

雑誌

Journal of Science and Technology in Lighting (ISSN:24323225)

巻号頁・発行日

vol.42, pp.29-32, 2019-03-27 (Released:2019-04-01)

参考文献数

12

被引用文献数

1

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Blue light emitting diode (LED) light is being used various devices for recent decades. Blue LED light has the 450–500 nm wavelengths, and high photon energy compared with green or red LED light. It is known that the exposure to blue LED light causes retinal photoreceptor cells damage. It is unknown whether the blue LED light cut particle containing lens has a protective effect against blue LED light-induced cell damage although the absorbance by colored lens shows a protective effect. Thus, the purpose of this study was to reveal that the protective effect of blue LED light cutting particle containing lens against photoreceptor and corneal epithelial cell damage induced by blue LED light exposure. We irradiated blue LED light to the mouse photoreceptor cells and human cornea epithelial cells with or without lens. The lens containing about one third blue LED light cutting particle (TECHPOLYMER) decreased production of reactive oxygen species and improved cell death rate and cell viability rate. These findings show that TECHPOLYMER containing lens may protect photoreceptor and cornea cells by reducing of reactive oxygen species.

著者

Kodai Ishida Tomohiro Yako Miruto Tanaka Wataru Otsu Shinsuke Nakamura Masamitsu Shimazawa Hideshi Tsusaki Hideaki Hara

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.44, no.7, pp.937-946, 2021-07-01 (Released:2021-07-01)

参考文献数

45

被引用文献数

4

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The corneal epithelium is continuously exposed to oxygen, light, and environmental substances. Excessive exposure to those stresses is thought to be a risk factor for eye diseases. Photokeratitis is damage to the corneal epithelium resulting in a painful eye condition caused by unprotected exposure to UV rays, usually from sunlight, and is often found in people who spend a long time outdoors. In modern life, human eyes are exposed to artificial light from light-emitting diode (LED) displays of computers and smartphones, and it has been shown that short-wavelength (blue) LED light can damage eyes, especially photoreceptors. However, the effect of blue LED light on the cornea is less understood. In addition, it is important to develop new treatments for preserving human eyesight and eye health from light stress. Here, we used human corneal epithelial cells-transformed (HCE-T) cells as an in-vitro model to investigate the protective effect of NSP-116, an imidazolyl aniline derivative, against the oxidative stress induced by light in the corneal epithelium. Treatment with 10 µM NSP-116 significantly increased the cell viability and reduced the death ratio following UV or blue LED light exposure. Furthermore, NSP-116 treatment decreased light-induced reactive oxygen species production and preserved the mitochondrial membrane potential. Immunoblotting data showed that NSP-116 suppressed the stress response pathway. Finally, NSP-116 treatment prevented corneal epithelial apoptosis induced by blue LED light in an in-vivo mouse model. In conclusion, NSP-116 has a protective effect against oxidative stress and corneal cell death from both UV and blue LED light exposure.