- 著者
- Erika Fujimoto Yumi Matsushita Toshihiro Nakajima Naoko Yagishita Tsutomu Yamasaki Tohru Nakanishi
- 出版者
- THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
- 雑誌
- Journal of Hard Tissue Biology (ISSN:13417649)
- 巻号頁・発行日
- vol.25, no.4, pp.377-382, 2016 (Released:2016-10-05)
- 参考文献数
- 18
- 被引用文献数
- 1 4
Tetraspanin CD81, which is up-regulated in rheumatoid arthritis (RA) synoviocytes, is a molecule containing four transmembrane domains. We recently showed that small interfering RNA targeting CD81 (CD81 siRNA) ameliorated arthritis in rats. In that study, the expression of synoviolin was decreased in RA joints by CD81 siRNA. We also showed that CD81 siRNA decreased the expression of synoviolin and TNF-α in SW982 synovial sarcoma cells, suggesting that overexpression of CD81 in synovial cells induces the expression of both synoviolin and TNF-α in the same cells. Here we raised monoclonal antibodies (MAbs) against CD81 by immunization of the outer membrane region of CD81 (LEL) that was overexpressed as a recombinant protein. One of these MAbs stimulated CD81 on the membrane of SW982 and up-regulated the expression of synoviolin. We also defined the region of the synoviolin gene promoter that is essential for induction of synoviolin gene expression by using reporter gene system. This system might be useful for the screening of anti-RA medicine.