著者
Shota Kodaira Yu-ki Tanaka Shoji Hayashi Shogo Aoki Takafumi Hirata Shinobu Ishigaki Kazumasa Aoki
出版者
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
雑誌
Journal of Hard Tissue Biology (ISSN:13417649)
巻号頁・発行日
vol.32, no.2, pp.127-132, 2023 (Released:2023-04-24)
参考文献数
27

Female birds have a medullary bone (MB) on the innermost surface of a cortical bone (CB) during the egg-laying cycle. This bone tissue is known to store calcium (Ca) for eggshell formation. As a result of Ca isotopic analyses of MB and CB from the mature female chickens using a multiple collector-ICP-mass spectrometer (ICP-MS), the 43Ca/42Ca and 44Ca/42Ca ratios of MB were higher than those of CB in the bones of the same individual. Moreover, elemental mapping of the CB and MB using a laser ablation-ICP-MS revealed that Mn, Zn, and Cu contents in the MB which are used for activating bone morphogenic protein were higher than those in CB. According to the mechanism of Ca isotope fractionation reported in our previous study, the difference in Ca isotope ratio between MB and CB can be explained by the change in bone turnover rate, i.e., the balance between bone formation and resorption. Therefore, the difference in Ca isotope ratio between MB and CB reflects the different Ca balances during the bone tissue formation of each part.
著者
Tetsuro Kono Arata Watanabe Takeshi Kanno Yukari Ootani Ryo Tamamura Toshiro Sakae Hiroyuki Okada
出版者
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
雑誌
Journal of Hard Tissue Biology (ISSN:13417649)
巻号頁・発行日
vol.28, no.2, pp.129-138, 2019 (Released:2019-04-24)
参考文献数
44

To elucidate the crystallo-chemical reaction between the enamel and carbonated soft drink, a micro-X-ray diffraction analysis (micro-XRD) was conducted using the human tooth sections soaked in the drink (Sprite®) for 1 and 7 days. In this study, 4 typically and heavily dissolved teeth were selected from 20 teeth, and the divergence of the crystallographic properties of the enamel was analyzed.All of the untreated human tooth enamels exhibited only the apatitic XRD patterns. We confirmed that the carbonated soft drink changed the macro- and microscopic morphological features of individual tooth enamel. The following 4 results were obtained from our study: i) the unit cell dimensions and crystallinity of the untreated enamel apatite varied between the layers and the individual teeth; ii) after the soaking experiment, the crystallographic properties of the enamels changed remarkably and displayed non-uniformity; iii) no relationship was discerned between the enamel’s crystallographic properties and the sensitivity of the carious attack; and iv) the intermediate reaction product of soaking was uncovered; however, it was not identified at this time. The presence or absence of occurrence and variations in the amount of intermediate product reflected the complex chemical and crystallo-chemical reactions between the decalcification solution and the human tooth enamel crystallites.We clearly established that the untreated human tooth enamels differed in their crystallographic properties and did not react in the same manner, which resulted in varied apatitic structures after soaking. These results necessitate reconsideration of the generally accepted caries protection methods that are applied as common standards for all individuals and tooth enamels.
著者
Rafiqul Islam Mohammad Khursheed Alam Ayako Mukai Yumiko Murakami Masahito Shoumura Naoto Osuga Mohd Fadhli Khamis
出版者
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
雑誌
Journal of Hard Tissue Biology (ISSN:13417649)
巻号頁・発行日
vol.26, no.4, pp.361-368, 2017-10-01 (Released:2017-10-03)
参考文献数
24
被引用文献数
1

Research related to tooth size, tooth size discrepancy and relationship with BMI in transgender population is yet to be explored in dentistry. The purpose of this study is to establish normative data on mesiodistal width dimensions associated with BMI and tooth size discrepancy in transgender population. The data were derived from dental casts of 151 transgender individuals (75 transgender male, 76 transgender female; age group 18-30). Data were analysed using independent t-test and ANOVA. The mean and standard deviation were calculated for individual tooth size, Bolton’s overall and anterior ratios, and BMI separately for transgender males and females. The mesiodistal widths of the maxillary teeth showed higher variability than the mandibular teeth and the mean value was higher in transgender females than in males and revealed statistically significant differences. Bolton’s anterior ratios were found to be 78.05 (±3.67) for male and 78.90 (±4.12) for female. The mean of Bolton’s overall tooth ratio for male 91.03(±3.66) and for women was 91.46(±3.91) with no significant differences (p >0.05). No significant differences between BMI and mesiodistal tooth dimensions except for the left first molar tooth in mandible and left lateral incisor tooth in maxilla between overweight and underweight groups (p<0.05). These findings indicate that population-specific standards are necessary for clinical assessments and for several dental treatment purposes. Moreover, it is appropriate to use transgender norms in a regular dental practice for transgender individuals.
著者
Bayarmaa Batzorig Kenjiro Nakano Kosei Murata Mayumi Maesako Kazuho Inoue Takafumi Kishimoto Shigetaka Tomoda Hatsuhiko Maeda Taku Horie Morioki Fujitani
出版者
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
雑誌
Journal of Hard Tissue Biology (ISSN:13417649)
巻号頁・発行日
vol.30, no.1, pp.19-26, 2021 (Released:2021-01-27)
参考文献数
29

In the treatment of dentin hypersensitivity accompanying tooth substance defects such as wedge-shape defects, hypoesthesia can be achieved by applying a desensitizing agent before carrying out restoration using resin composite. However, almost no research has investigated the adhesion of resin to dentin coated with the latest desensitizing agents. Therefore, this study investigated the effects of various desensitizing agents on the adhesion of resin to dentin in combination with a 1-step self-etch system by using a hypersensitive dentin model in which the dentinal tubules were opened without etching and there was almost no smear layer on the intertubular dentin. Specimens with a #4000 polished dentin flat surface were ultrasonically cleaned for 60 min (15 min × 4 times). Then, the bond strength, failure modes, and micromorphology of surfaces coated with desensitizing agent to which resin was bonded immediately afterward and surfaces coated with desensitizing agent to which the resin was bonded after storage for 7 days in water were compared against a control to which no desensitizing agent was applied. The desensitizing agents used in this research did not promote adhesion of the resin immediately after application, but rather suppressed or completely obstructed it. Although deposits of microparticles and thin film material, which were observed immediately after application, tended to disappear after 7 days of storage in water, some of the desensitizing agents exhibited the same bond strength as the control, whereas other desensitizing agents did not show recovery of adhesion strength. Therefore, care is required when performing resin restoration immediately after application of a desensitizing agent, depending on the agent used, and caution must be exercised in the selection of desensitizing agents in the clinical setting.
著者
Noboru Ishikawa Yuzo Hirayama Yasuo Miake Kei Kitamura Norio Kasahara Shinichi Abe Hitoshi Yamamoto
出版者
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
雑誌
Journal of Hard Tissue Biology (ISSN:13417649)
巻号頁・発行日
vol.28, no.3, pp.289-296, 2019 (Released:2019-07-31)
参考文献数
29
被引用文献数
2

In the field of forensic odontology, not only personal identification using oral conditions, including dental treatment marks and DNA typing, but also species identification, age or sex estimation using cranial or partial bones, and time after death may also be applicable as estimation methods. Among these many tasks, one of the most difficult is species identification of fragmented calvarium. This is because the calvarium has poor morphological features, except that it is a flat bone, and few reports have described how to differentiate it. In this study, a simple identification method for turtle shell (carapace and plastron) whose morphological characteristics closely resembled those of the calvarium was applied. As a result, in an enlarged image obtained using a stereoscopic microscope, the characteristics of each suture pattern could be confirmed. In the decalcified and non-decalcified bone specimens, the difference in the inner/outer laminar structure and the specific structural difference of the cancellous bone-equivalent part were confirmed. Furthermore, most of the features could be obtained in the destructive inspection when it was examined using micro CT imaging to determine whether discrimination by nondestructive inspection was possible or not. No significant difference in ingredients was found using the Electron Probe X-ray Micro Analyzer. The calvarium was more calcified than the carapace but less calcified than the plastron. From these results, we suggest that micro CT imaging is effective for discriminating between the calvarium and turtle shell in a short time.
著者
Erika Fujimoto Yumi Matsushita Toshihiro Nakajima Naoko Yagishita Tsutomu Yamasaki Tohru Nakanishi
出版者
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
雑誌
Journal of Hard Tissue Biology (ISSN:13417649)
巻号頁・発行日
vol.25, no.4, pp.377-382, 2016 (Released:2016-10-05)
参考文献数
18
被引用文献数
1 4

Tetraspanin CD81, which is up-regulated in rheumatoid arthritis (RA) synoviocytes, is a molecule containing four transmembrane domains. We recently showed that small interfering RNA targeting CD81 (CD81 siRNA) ameliorated arthritis in rats. In that study, the expression of synoviolin was decreased in RA joints by CD81 siRNA. We also showed that CD81 siRNA decreased the expression of synoviolin and TNF-α in SW982 synovial sarcoma cells, suggesting that overexpression of CD81 in synovial cells induces the expression of both synoviolin and TNF-α in the same cells. Here we raised monoclonal antibodies (MAbs) against CD81 by immunization of the outer membrane region of CD81 (LEL) that was overexpressed as a recombinant protein. One of these MAbs stimulated CD81 on the membrane of SW982 and up-regulated the expression of synoviolin. We also defined the region of the synoviolin gene promoter that is essential for induction of synoviolin gene expression by using reporter gene system. This system might be useful for the screening of anti-RA medicine.