著者

Hubing Zhao Yingyu Huo Gangren Yang Xiaozhu Liu Zhongtai Li Diao Luo Xiaoyu Zhao Xu Li

出版者

Japanese Society for Food Science and Technology

雑誌

Food Science and Technology Research (ISSN:13446606)

巻号頁・発行日

pp.FSTR-D-23-00033, (Released:2023-08-11)

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In order to solve the abnormal fermentation problems caused by the slow and difficult temperature rise in the saccharification piling during the fermentation of sauce-flavor baijiu under low temperatures in winter. A special oxygen dissolving device made of stainless steel was applied in the second sorghum input of sauce-flavor baijiu, as well as the 1th and 2th rounds of saccharification piling fermentation in this study. In addition, its effects on the saccharification piling temperature, the 1 th and 2th rounds of physical and chemical indicators, microbial population, and flavor components are analyzed. The results indicate that the use of a stainless steel oxygen dissolver significantly increased the "piling core" temperature of a saccharification piling and that this improvement was highly correlated with the oxygen dissolver. Additionally, it decreased the water content, sugar concentration, and acidity of the "piling core" of the saccharification piling, all of which were negatively correlated with the height of the dissolved oxygen reactor. Furthermore, the stainless steel special dissolved oxygen device also facilitated the growth and reproduction of saccharification piling microorganisms, particularly yeast. The use of the stainless steel special dissolved oxygen device, therefore, resulted in an increase in the concentration of acetaldehyde, ethyl acetate, propanol, isoamyl alcohol, β-phenylethanol, ethyl palmitate, and other flavoring compounds. Accordingly, the stainless steel special oxygen dissolving device demonstrates a significant application potential in the 1th and 2th rounds of saccharification piling fermentation of sauce-flavor baijiu.

著者

Rina Miyake Tomohiro Uchimura Xu Li Totaro Imasaka

出版者

公益社団法人日本薬学会

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.61, no.1, pp.82-84, 2013-01-01 (Released:2013-01-08)

参考文献数

13

被引用文献数

2

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Fluorescence lifetime imaging microscopy (FLIM) was used to monitor the interaction between androgen receptor (AR) tagging of a green fluorescent protein (GFP) and the ligands in living cells. The fluorescence lifetime of the AR-GFP without ligands was ca. 3.1 ns, which was reduced to ca. 2.5 ns after treatment with agonist 5α-dihydrotestosterone. On the other hand, the fluorescence lifetime of AR-GFP was not changed after treatment with antagonist hydroxyflutamide. The reaction kinetics was simulated in the present study, and the obtained results indicated the possibility of the presence of an intermediate complex during the reaction. FLIM can be used to record the ratio of the AR as it reacts with an agonist, and, therefore, it is useful for acquiring information concerning the interaction between AR and ligands in living cells.