- 著者
- Y-HTaguchi
- 雑誌
- 研究報告バイオ情報学(BIO)
- 巻号頁・発行日
- vol.2012-BIO-28, no.3, pp.1-6, 2012-03-21
MiRNAs are recently known to be critical players causing cell senescence, by regulating target genes. Thus inference of miRNAs critically regulating target genes is important. However, miRNAs critically regulating target genes are believed to have significant fold changes, typically upregulations, during cell senescence. In this study, we consider the target gene regulation by miRNAs together with miRNAs expression change during fibroblast IMR90 cell senescence. Then we found that the simultaneous consideration of two criterion lists more feasible miRNAs: i.e., miRNAs being more often reported to be down/upregulated and/or having biological backgrounds inducing cell senescence. Thus, the amount of target gene regulation, which can be inferred by the recently developed MiRaGE Server, is recommended to be considered together for the estimation of miRNAs critically contributing to cell senescence.
- 著者
- Y-HTaguchi Akira Okamoto
- 雑誌
- 研究報告バイオ情報学(BIO)
- 巻号頁・発行日
- vol.2012-BIO-28, no.16, pp.1-6, 2012-03-21
Proteomic analysis is very useful procedure to understand the bacterial behavior changes with reaction to the external environment. This is because most of genomic information of bacteria is devoted to code enzyme to control metabolic networks inside the individual cell. In this paper, we have performed proteomic analysis of Streptococcus pyogenes, which is known to be a flesh-eating bacteria and can cause several human life-threatening diseases. Its proteome during growth phase is measured for four time points under two different incubation conditions; with and without shaking. The purpose of it is to understand adaptivity to oxidative stress. Principal component analysis is applied and turns out to be useful to depict biologically important proteins for both supernatant and cell components.