著者

Yamauchi Daisuke

出版者

日本植物細胞分子生物学会

雑誌

Plant biotechnology (ISSN:13424580)

巻号頁・発行日

vol.17, no.3, pp.263-267, 2000-09-01

参考文献数

20

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A gene for Brazil nut 2S albumin, a methionine-rich protein, was fused to a promoter region of the canavalin gene. This chimeric gene was introduced into shoot apices of embryonic axes of French bean by particle bombardment and seeds were obtained from plants regenerated from the bombarded axes. Protein in the transgenic seeds was analyzed by immunoblotting with an antiserum against Brazil nut 2S albumin. A 12-kDa immunoreactive polypeptide was detected and its amount in the seed was estimated to comprise less than 1% of the soluble protein.