著者
Mari Sugiyama Miyu Norizuki Shinji Kikuchi Yasuo Yasui Katsuhiro Matsui
出版者
Japanese Society of Breeding
雑誌
Breeding Science (ISSN:13447610)
巻号頁・発行日
vol.73, no.2, pp.230-236, 2023 (Released:2023-06-06)
参考文献数
27
被引用文献数
1

Common buckwheat (Fagopyrum esculentum) is an annual self-incompatible plant that is widely grown. The genus Fagopyrum comprises more than 20 species, including F. cymosum, a perennial that, unlike common buckwheat, is highly resistant to excess water. In this study, we developed interspecific hybrids between F. esculentum and F. cymosum via embryo rescue, to improve undesirable traits of common buckwheat, such as low tolerance to excess water. The interspecific hybrids were confirmed by genomic in situ hybridization (GISH). We also developed DNA markers to confirm the identity of the hybrids and if genes derived from each genome were inherited by the next generation. Observations of pollen indicated that the interspecific hybrids were essentially sterile. Unpaired chromosomes and abnormal segregation during meiosis were likely responsible for the pollen sterility of the hybrids. These findings could facilitate buckwheat breeding to produce lines that can withstand harsh environments with wild or related species in the genus Fagopyrum.
著者
Bibian Diway Yasuo Yasui Hideki Innan Yayoi Takeuchi
出版者
JAPAN SOCIETY OF TROPICAL ECOLOGY
雑誌
Tropics (ISSN:0917415X)
巻号頁・発行日
vol.30, no.4, pp.71-82, 2022-03-01 (Released:2022-03-01)
参考文献数
37

A new Rafflesia population was found in Naha Jaley, Sarawak, in 2012. This study aimed to identify this Rafflesia species and investigate its bud growth. First, we described the flower characteristics and compared them with Rafflesia keithii and R. tuan-mudae, which are candidate species for the flower. Also, we investigated the phylogenetic position of this Rafflesia within Rafflesiaceae using DNA analysis. To estimate bud growth curve, we observed bud development from April 2013 to November 2013 in the field. Based on morphological comparisons and phylogenetic analysis, we confirmed the newly discovered population of the Rafflesia at Naha Jaley was R. tuan-mudae, which is the new locality of the species at the most Eastern side of the known distribution for this species. The results indicated that absolute growth rate was greater in larger buds. We also estimated that it took a year to bloom from the initial bud stage. The mortality in one of our sites was very high, with more than 80 % of buds dead prior to flower opening. This flower would be vulnerable to extinction due to their extraordinary characteristics and anthropogenetic effects. Finally, we discussed how Rafflesia populations in Naha Jaley can be conserved both in-situ and ex-situ.