著者

Mao OTAKE Yoshiaki UKITA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.35, no.10, pp.1123-1127, 2019-10-10 (Released:2019-10-10)

参考文献数

23

被引用文献数

1

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Measurement of single molecule interaction is performed by various micromanipulation techniques. However, a complex operation is required to perform the measurement, so the measurement throughput is low and a large amount of time is required for statistical analysis. In this paper, we propose a method for measuring intermolecular interactions that applies a tensile force to the intermolecular bond by using centrifugal force and can easily measure binding force. The binding force of an antigen–antibody reaction system was measured by the use of the proposed method as a proof of concept. The measured binding forces were distributed in the range of 19 – 133 pN. The values obtained by use of the proposed method were consistent with the conventional method. This result is promising for applications that require further development, such as the development of a biosensor as a novel measuring technique that is based on the intermolecular interaction measurement.