- 著者
- Retori Hiraoka Hirohiko Teishima Teruhiro Takabe Teruaki Suzuki Yoshito Tanaka
- 出版者
- Carcinological Society of Japan
- 雑誌
- Crustacean Research (ISSN:02873478)
- 巻号頁・発行日
- vol.50, pp.9-15, 2021 (Released:2020-12-24)
- 参考文献数
- 32
- 被引用文献数
- 1
The nonnative freshwater shrimp Palaemon sinensis (Sollaud, 1911) has been reported at various locations in Japan. This study compared environmental DNA (eDNA) analysis with conventional capturing and investigation as a method to detect P. sinensis in the Tama River. We developed species-specific primers to amplify a 225-bp fragment of the cytochrome c oxidase subunit I gene for eDNA analysis. The invasive shrimps were detected using eDNA analysis in all areas where they were observed using the capture and investigation method. Thus, eDNA analysis can be applied to detect sparsely distributed P. sinensis in the Tama River, making it a useful method to detect invasive shrimps and to help prevent ecological disruption.
- 著者
- Masaya Matsuki Nobuhiro Shimizu Kazuhiro Tobiishi Yoshito Tanaka Haruyo Yamaguchi Tomoharu Sano
- 出版者
- Japan Society on Water Environment
- 雑誌
- Journal of Water and Environment Technology (ISSN:13482165)
- 巻号頁・発行日
- vol.20, no.6, pp.261-272, 2022 (Released:2022-12-10)
- 参考文献数
- 34
Cyanotoxins produced by blue-green algae in lakes are among the most serious threats to water quality worldwide. As global warming rapidly extends the locations and timing of blue-green algae blooms, a simple and accessible method for the detection and quantification of cyanotoxins in fresh water is increasingly necessary. Here, a quick, simple and accessible simultaneous analytical method for five cyanotoxins (cylindrospermopsin, anatoxin-a, microcystin-RR, YR and LR) is reported. This method has three advantages. First, it does not require complicated operations, such as a concentration operation. Second, it employs an HPLC column without high pressure. Third, the use of stable isotope-labeled surrogates enables correct identification and precise quantification of cyanotoxins. The method was applied to the lakes of Fukuoka Prefecture in Japan, and four of the five above-named cyanotoxins (i.e., all but cylindrospermopsin) were detected. The limits of quantification were 20–43 ng/L, which were considerably lower than the WHO guideline values. The recovery levels were 97–104%. Microbial flora analysis revealed that the sources of anatoxin-a were Pseudanabaena limnetica and Cuspidothrix issatschenkoi, and the source of microcystins was the group A1 of Microcystis aeruginosa. This study provides a quick, easy and accessible method for the worldwide monitoring of cyanotoxin levels.