著者

Kei KONDOH Ayman ATIBA Kiyoshi NAGASE Shizuko OGAWA Takashi MIWA Teruya KATSUMATA Hiroshi UENO Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0279, (Released:2015-04-04)

被引用文献数

1

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In the present study, we compare a new carbon dioxide (CO2) absorbent, Yabashi lime® with a conventional CO2 absorbent, Sodasorb® as a control CO2 absorbent for Compound A (CA) and Carbon monoxide (CO) productions. Four dogs were anesthetized with sevoflurane. Each dog was anesthetized with four preparations, Yabashi lime® with high or low-flow rate of oxygen and control CO2 absorbent with high or low-flow rate. CA and CO concentrations in the anesthetic circuit, canister temperature, and carbooxyhemoglobin (COHb) concentration in the blood were measured. Yabashi lime® did not produce CA. Control CO2 absorbent generated CA and its concentration was significantly higher in low-flow rate than a high-flow rate. CO was generated only in low-flow rate groups, but there was no significance between Yabashi lime® groups and control CO2 absorbent groups. However, the CO concentration in the circuit could not be detected (≤5 ppm) and no change was found in COHb level. Canister temperature was significantly higher in low-flow rate groups than high-flow rate groups. Furthermore, in low-flow rate groups, the lower layer of canister temperature in control CO2 absorbent group was significantly higher than Yabashi lime® group. CA and CO productions are thought to be related to the composition of CO2 absorbent, flow rate, and canister temperature. Though CO concentration is equal, it might be safer to use Yabashi lime® with sevoflurane anesthesia in dogs than conventional CO2 absorbent at the point of CA production.

著者

Masaaki KATAYAMA Taku USHIO Shunsuke SHIMAMURA Yasuhiko OKAMURA Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0101, (Released:2014-08-05)

被引用文献数

1 3

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Tacrolimus (Tac) is a core immunosuppressive drug in human organ transplantation. In feline kidney transplantation, however, the cost of Tac therapy is a significant obstacle. Clarithromycin (CLM) increases the blood trough level of Tac, effectively reducing the Tac dosage in human transplant patients. The interaction between CLM and Tac in cats has not been reported. In this study, the effect of multiple CLM dosing on the pharmacokinetics of Tac in three healthy cats was investigated. The treatments included Tac at 0.3 mg/kg and Tac at 0.3 mg/kg + multiple-dose CLM at 10 mg/kg. Co-administration of CLM and Tac resulted in significant increases in the oral bioavailability of Tac. These preliminary findings suggest that administration of multiple doses of CLM may decrease the required Tac dosage in Tac-based immunosuppressive therapy used as an alternative to the classic cyclosporine-based protocol for feline renal transplantation.

著者

Mohammed ABDEL-HAKIEM Ayuko YAMASHITA Ayman ATIBA Yasuhiko OKAMURA Masaaki KATAYAMA Haroun YOUSSEF Hiroshi ISOMURA Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0233, (Released:2015-08-21)

被引用文献数

1

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The pathogenesis of intervertebral disc (IVD) degeneration is not fully understood. The biomolecular signaling pathways involved in the IVD degeneration require further investigation. The aim of this study was to investigate the expression of NG2 proteoglycan in the degenerated IVD. IVD samples were obtained from 16 Dachshunds that were confirmed to have IVD herniation and subsequently underwent hemilaminectomy. The samples were subjected to histological and immunohistochemical (IHC) examination. IHC revealed positive results for the expression of NG2 proteoglycan in all examined samples. The results showed the expression of NG2 proteoglycan by the degenerated IVDs.

著者

Ayman ATIBA Hiroshi UENO Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.73, no.5, pp.583-589, 2011 (Released:2011-05-31)

参考文献数

32

被引用文献数

11 55

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Delayed wound healing is one of the complications of diabetes mellitus. The present study was performed to investigate the effect of Aloe vera oral administration on open wounds in type 2 diabetic rats. Full thickness open wounds (1.5 × 1.5 cm) were created under general anesthesia on the backs of the rats. These rats were divided into two group, a control group (Group C) and an Aloe vera oral administration group (Group A). Each wound area was measured on days 1, 2, 4 and 8 postwounding. The stages of wound granulation tissues were evaluated histopathologically. The expression of transforming growth factor (TGF)-β1 and vascular endothelial growth factor (VEGF) were determined by immunohistochemically. The wounds were significantly contracted in Group A on days 2, 4 and 8 postwounding. Histological results revealed that the inflammatory cell infiltration, angiogenesis, extracellular matrix deposition and epithelialization were promoted in Group A, respectively. The immunohistochemical results revealed that both TGF-β1 and VEGF protein-positive cells increased in Group A on day 4 postwounding. We concluded that Aloe vera oral administration accelerated wound healing in type 2 diabetic rats.

著者

Tadahisa MASHITA Hiroaki KAMISHINA Yuya NAKAMOTO Yosuke AKAGI Ataru NAKANISHI Yusuke HARASAKI Tsuyoshi OZAWA Takashi UEMURA Yui KOBATAKE Shunsuke SHIMAMURA Naoki KITAMURA Sadatoshi MAEDA Yuji UZUKA Gerry SHAW Jun YASUDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0582, (Released:2014-12-19)

被引用文献数

4

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The aim of this study was to evaluate the prognostic value of concurrent measurement of serum phosphorylated neurofilament heavy subunit (pNF-H) concentration and intramedullary T2W hyperintensity in parapalegic to paraplegic dogs. Our hypothesis was that concurrent measurement of these would provide a more accurate prediction of functional outcome in dogs with thoracolumbar intervertebral disc herniation (IVDH). A prospective case-control clinical study was designed using 94 dogs with acute onset of thoracolumbar IVDH. The association of serum pNF-H concentration, T2W hyperintensity on sagittal MRI (T2H/L2), deep pain perception and surgical outcome were evaluated with logistic regression analysis after three months for all 94 surgically treated dogs. Sensitivity to predict non-ambulatory outcome was compared among pNF-H and T2H/L2 and combination of both. Logistic regression analysis indicated that serum pNF-H concentration and T2H/L2 were significantly correlated with surgical outcome (P<0.05); however, deep pain perception was not (P=0.41). The results of logistic regression analysis indicated that the odds ratios of unsuccessful long-term outcome were 2.6 for serum pNF-H concentration, 1.9 for T2H/L2 and 2.3 for deep pain sensation. The sensitivity and specificity to predict non-ambulatory outcome for using serum parameter pNF-H>2.6 ng/ml, using T2H/L2 value of >0.84 and using both serum pNF-H and T2H/L2, were 95% and 75.7%, 65% and 86.5%, and 90.0% and 97.5%, respectively. Therefore, combined measurements of serum pNF-H and T2H/L2 might be useful for predicting long-term outcome in dogs with thoracolumbar IVDH.

著者

Masaaki KATAYAMA Shinobu TSUCHIAKA Tomoki MOTEGI Masao MIYAZAKI Tetsuro YAMASHITA Shunsuke SHIMAMURA Yasuhiko OKAMURA Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0565, (Released:2014-03-05)

被引用文献数

2

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Phosphate-buffered sucrose (PBSc) solution is effective for short-term hypothermic preservation of tissue during feline kidney transplantation. A high-molecular-weight polyethylene glycol (35,000 Da, PEG35) reportedly enhanced the protective effects against cold-induced tubular injuries in animal kidney transplantation models. We investigated the ability of PBSc solution containing PEG35 to preserve cultured feline kidney cells using in vitro WST-8 cell proliferation assays. PEG35 significantly improved cell viability during 24 hr of cold preservation. PBSc containing 20 g/l PEG35 achieved an effect almost equal to that of University of Wisconsin (UW) solution, the gold standard preservation solution used in human clinical kidney transplantation, for up to 24 hr of preservation. Our results suggest that PBSc containing PEG35 provides an excellent medium for graft cold storage during feline kidney transplantation.

著者

Masaaki KATAYAMA Yoshiki KAWAKAMI Rieko KATAYAMA Shunsuke SHIMAMURA Yasuhiko OKAMURA Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0209, (Released:2013-11-05)

被引用文献数

3

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Clarithromycin (CLM) has been known to increase the cyclosporine (CsA) trough level in human and feline organ transplant patients. However, the interaction of CLM with CsA has not been reported in dogs. In this study, the effects of multiple dosing of CLM on the pharmacokinetics of CsA in three healthy beagles were investigated. The treatments included CsA 10 mg/kg alone and CsA 10 mg/kg + multiple-dose of CLM 10 mg/kg. Co-administration of CLM with CsA resulted in significant increases of oral bioavailability of CsA. The results of our study suggest that administration of multiple therapeutic doses of CLM may decrease the required CsA dosage in CsA-based immunosuppressive therapy in renal transplanted dogs.

著者

Yasunori SATO Shunsuke SHIMAMURA Tadahisa MASHITA Saori KOBAYASHI Yasuhiko OKAMURA Masaaki KATAYAMA Hiroaki KAMISHINA Reeko SATO Yuji UZUKA Jun YASUDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.7, pp.949-953, 2013 (Released:2013-07-31)

参考文献数

17

被引用文献数

5 22

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In humans, increased levels of GFAP in the CSF and blood have been reported with various neural diseases. However, there has been no study describing the usefulness of GFAP in the blood for disease of the spinal cord in dogs. The aim of this study was to describe the utility of GFAP in serum for a diagnosis of progressive myelomalacia. Fifty-six dogs with acute thoracolumbar IVDD diagnosed by computed tomography with myelography or MRI were included. Serum specimens were collected at initial presentation from all cases and at follow-up examinations from some cases. Serum samples were assayed for GFAP concentrations using a commercially available GFAP ELISA Kit. Progressive myelomalacia was the final diagnosis in 8/51 cases (15.6%). Eight dogs had clinical signs suggestive of progressive myelomalacia, of which 6 were positive and 2 were negative by GFAP. Seven dogs had a detectable level of serum GFAP, of which 6 had the onset of progressive myelomalacia. The sensitivity and specificity of the GFAP to progressive myelomalacia were 75% and 97.7%, respectively. The results suggest the utility of GFAP in serum in the diagnosis of progressive myelomalacia.

著者

Yasunori SATO Shunsuke SHIMAMURA Tadahisa MASHITA Saori KOBAYASHI Yasuhiko OKAMURA Masaaki KATAYAMA Hiroaki KAMISHINA Reeko SATO Yuji UZUKA Jun YASUDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0483, (Released:2013-03-08)

被引用文献数

5 22

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In humans, increased levels of GFAP in the CSF and blood have been reported with various neural diseases. However there has been no study describing the usefulness of GFAP in the blood for disease of the spinal cord in dogs. To describe the utility of GFAP in serum for a diagnosis of progressive myelomalacia. Fifty-six dogs with acute thoracolumbar IVDD diagnosed by computed tomography with myelography or MRI were included. Serum specimens were collected at initial presentation from all cases and at follow-up examinations from some cases. Serum samples were assayed for GFAP concentrations using a commercially available GFAP ELISA KIT. Progressive myelomalacia was the final diagnosis in 8/52 (15.3%). Eight dogs had clinical signs suggestive of progressive myelomalacia, of which 6 were positive and 2 were negative by GFAP. Seven dogs had a detectable level of serum GFAP, of which 6 had the onset of progressive myelomalacia. The sensitivity and specificity of the GFAP to progressive myelomalacia were 75 and 97.7%, respectively. The results suggest the utility of GFAP in serum in the diagnosis of progressive myelomalacia.