著者

Jackie Lou TAGUBASE Shigeaki UENO Yumiko YOSHIE Tetsuya ARAKI

出版者

Japan Society of Refrigerating and Air Conditioning Engineers

雑誌

日本冷凍空調学会論文集 (ISSN:13444905)

巻号頁・発行日

pp.16-17NR_OA, (Released:2016-08-31)

参考文献数

26

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The effect of freezing, and iced and hot water thawing on the quality of durian pulp was investigated. Mature durian pulp was removed with seeds, vacuum-packed, and frozen at -20°C. Thawing in iced water (~0°C) and hot water (~90°C) was then applied and the quality of the pulp was assessed based on physicochemical properties (pH, moisture content, soluble solids concentration (SSC), color, sugar content (sucrose, glucose, and fructose), and organic acid content (succinic acid and citric acid ), texture and smell profile. Overall, the freezing and thawing conditions, particularly the hot water thawing, posed an effect to the moisture content, color, and smell profile of the durian pulp. A significant increase in the moisture content, as well as a decrease in the color brightness was observed. Furthermore, the hot water-thawing process also induced slight variation to the smell attribute and strength of the entirety of smell. Although the sugar content significantly decreased after freezing and thawing, it was only affected by the freezing process but not by the thawing conditions. No significant variations were noted in the pH, SSC, organic acids and texture of the frozen durian pulp.

著者

Mariko OGUSHI Yumiko YOSHIE-STARK Takeshi SUZUKI

出版者

Japanese Society for Food Science and Technology

雑誌

Food Science and Technology Research (ISSN:13446606)

巻号頁・発行日

vol.11, no.2, pp.202-206, 2005 (Released:2006-11-08)

参考文献数

22

被引用文献数

6 15

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Sea cucumber is a traditional food item in Asia and has been reported to exhibit antifungal, antitumor, and antioxidant bioactivity as well as other properties. In this study, sea cucumbers were treated with hot water at 98°C for 60min to prepare extracts that were then used to assess their effect on the proliferation and H2O2 susceptibility of human colon adenocarcinoma Caco-2 cells. The growth of Caco-2 cells was significantly inhibited by sea cucumber extracts in a dose dependent manner. Cell proliferation was inhibited by high molecular weight sea cucumber extract at a concentration of 0.108mg/mL. No growth was observed at 1.04mg/mL of the hot water extract after 96h incubation. Cell damage by sea cucumber extract was evident above 1mg/mL. H2O2 showed concentration dependent cytotoxity to Caco-2 cells. In addition, coadministration of sea cucumber extracts intensified the H2O2 cytotoxity.

著者

Mariko OGUSHI Yumiko YOSHIE-STARK Takeshi SUZUKI

出版者

Japanese Society for Food Science and Technology

雑誌

Food Science and Technology Research (ISSN:13446606)

巻号頁・発行日

vol.12, no.4, pp.290-294, 2006 (Released:2007-10-05)

参考文献数

13

被引用文献数

3 6

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To study the anti-tumor activity of sea cucumber extracts, human colon adenocarcinoma Caco-2 cells were exposed to hot water extract of sea cucumber. Morphological changes with apoptotic bodies were observed in the cells treated with the extract. We demonstrated the induction of apoptosis using phosphatidylserine translocation (APOPercentage Assay kit), terminal deoxynucleotide transferase-mediated dUTP-biotin nick-end labeling (TUNEL), and DNA fragmentation observed as DNA ladder. These results show that apoptosis was induced by a high molecular weight fraction in a dose dependent manner. These data suggest that water soluble and high molecular weight compounds from sea cucumber might exhibit anti-tumor activity by triggering apoptosis. The apoptosis-inducing activity may contribute to cancer chemopreventive effects of sea cucumber.