- 著者
- Yusuke KITAMURA Kotaro MISHIO Pelin ARSLAN Boui IKEDA Chiharu IMOTO Yousuke KATSUDA Toshihiro IHARA
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.36, no.8, pp.959-964, 2020-08-10 (Released:2020-08-10)
- 参考文献数
- 12
- 被引用文献数
- 6
Ferrocene (Fc) and β-cyclodextrin (βCyD) were modified at each end of stem-loop structured DNA as an electrochemical signal generator and its quencher, respectively, to give an electrochemical molecular beacon (eMB). A relatively high efficiency of signal quenching was achieved by an inclusion complex (βCyD ⊃ Fc) formation that was induced on the stem structure of the closed form (= stem-loop structure) of eMB. With the addition of target DNA, the structure of eMB opened to form a linear duplex, where the Fc dissociated from the βCyD to restore its intrinsic electrochemical signal. The signal contrast of the electric current for this off/on-type sensor was high, ca. 95. This technique did not require any modification of the electrode surface, and it realized the detection of the target nucleic acids in a homogeneous solution with a high sensitivity using high-performance liquid chromatography (HPLC) equipped with electrochemical detector.
- 著者
- Yusuke KITAMURA Takaaki TANIGUCHI Miwako TSUTSUMI Leanddas NURDIWIJAYANTO Tomoya MATSUO Yousuke KATSUDA Toshihiro IHARA
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.36, no.4, pp.397-400, 2020-04-10 (Released:2020-04-10)
- 参考文献数
- 15
- 被引用文献数
- 1
A fluorescent dye-labeled DNA probe was adsorbed and quenched on the monolayer of RuO2 nanosheets. Significant fluorescent recovery was observed upon the addition of complementary DNA due to desorption of the probe from the surface of the RuO2 nanosheet through duplex formation. The efficiency of fluorescence recovery was higher than that for graphene oxide, which was known as a quencher-free platform for the detection of nucleic acids in a homogeneous solution.