- 著者
- Masahiro YAMASAKI Yoshihiro TSUBOI Yusuke TANIYAMA Naohiro UCHIDA Reeko SATO Kensuke NAKAMURA Hiroshi OHTA Mitsuyoshi TAKIGUCHI
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.16-0027, (Released:2016-04-28)
- 被引用文献数
- 3
The Babesia gibsoni heat shock protein 90 (BgHSP90) gene was cloned and sequenced. The length of the gene was 2,610 bp with two introns. This gene was amplified from cDNA corresponding to full length cording sequence (CDS) with an open reading frame of 2,148 bp. A phylogenetic analysis of the CDS of HSP90 gene showed that B. gibsoni was most closely related to B. bovis and Babesia sp. BQ1/Lintan and lies within a phylogenetic cluster of protozoa. Moreover, mRNA transcription profile for BgHSP90 exposed to high temperature were examined by quantitative real-time reverse transcription-polymerase chain reaction. BgHSP90 levels was elevated when the parasites were incubated at 43°C for 1 hr.