著者

Watari Hiromi Nakajima Hiroya Atsuumi Wataru Nakamura Takanori Nanya Takeshi Ise Yuji Sakai Ryuichi

出版者

Elsevier

雑誌

Comparative biochemistry and physiology Part C : Toxicology & pharmacology (ISSN:15320456)

巻号頁・発行日

vol.221, pp.82-88, 2019-07

被引用文献数

7

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We screened 868 marine extracts in search of hematopoietic molecules resulted in findings of several extracts that proliferated Ba/F3-HuMpl cells but not the cells expressed with other hematopoietic cytokine receptors, EPO and G-CSF. Separation of the most potent extract of a Micronesian sponge Corticium sp., guided by the cell proliferation assay using Ba/F3-HuMpl cells resulted in an isolation of thrombocorticin (ThC), a novel 14 kDa protein as an active principal. ThC displayed concentration-dependent proliferation of Ba/F3-HuMpl cells, and had a stronger activity than that of eltrombopag, a small molecule drug used to treat thrombocytopenia. ThC induced phosphorylation of STAT5, suggesting that it activates Jak/STAT pathway as in the case of TPO. These results together indicated that ThC is a specific agonist for c-Mpl, although the size and shape differs largely from TPO. Here we present isolation, characterization and biological activity of ThC.