著者
Watari Hiromi Nakajima Hiroya Atsuumi Wataru Nakamura Takanori Nanya Takeshi Ise Yuji Sakai Ryuichi
出版者
Elsevier
雑誌
Comparative biochemistry and physiology Part C : Toxicology & pharmacology (ISSN:15320456)
巻号頁・発行日
vol.221, pp.82-88, 2019-07
被引用文献数
7

We screened 868 marine extracts in search of hematopoietic molecules resulted in findings of several extracts that proliferated Ba/F3-HuMpl cells but not the cells expressed with other hematopoietic cytokine receptors, EPO and G-CSF. Separation of the most potent extract of a Micronesian sponge Corticium sp., guided by the cell proliferation assay using Ba/F3-HuMpl cells resulted in an isolation of thrombocorticin (ThC), a novel 14 kDa protein as an active principal. ThC displayed concentration-dependent proliferation of Ba/F3-HuMpl cells, and had a stronger activity than that of eltrombopag, a small molecule drug used to treat thrombocytopenia. ThC induced phosphorylation of STAT5, suggesting that it activates Jak/STAT pathway as in the case of TPO. These results together indicated that ThC is a specific agonist for c-Mpl, although the size and shape differs largely from TPO. Here we present isolation, characterization and biological activity of ThC.

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5本目は大学院生、辺浩美さんの造血サイトカイン受容体の論文。 "A novel sponge-derived protein thrombocorticin is a new agonist for thrombopoietin receptor" https://t.co/1AvUxsSuQ6 #oaweek2020

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